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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Erwinia tasmaniensis DNA repair protein recO (recO) CSB-YP455098ENB
CSB-EP455098ENB
CSB-BP455098ENB
CSB-MP455098ENB
CSB-EP455098ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UvrABC system protein C (uvrC) CSB-YP455099ENB
CSB-EP455099ENB
CSB-BP455099ENB
CSB-MP455099ENB
CSB-EP455099ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0352 protein ETA_12580 (ETA_12580) CSB-YP455100ENB
CSB-EP455100ENB
CSB-BP455100ENB
CSB-MP455100ENB
CSB-EP455100ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Acetate kinase (ackA) CSB-YP455101ENB
CSB-EP455101ENB
CSB-BP455101ENB
CSB-MP455101ENB
CSB-EP455101ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Shikimate kinase 2 (aroL) CSB-YP455102ENB
CSB-EP455102ENB
CSB-BP455102ENB
CSB-MP455102ENB
CSB-EP455102ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0229 protein ETA_15540 (ETA_15540) CSB-YP455103ENB
CSB-EP455103ENB
CSB-BP455103ENB
CSB-MP455103ENB
CSB-EP455103ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis D-amino acid dehydrogenase small subunit (dadA) CSB-YP455104ENB
CSB-EP455104ENB
CSB-BP455104ENB
CSB-MP455104ENB
CSB-EP455104ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0181 protein ETA_15280 (ETA_15280) CSB-YP455105ENB
CSB-EP455105ENB
CSB-BP455105ENB
CSB-MP455105ENB
CSB-EP455105ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Probable transcriptional regulatory protein ETA_14870 (ETA_14870) CSB-YP455106ENB
CSB-EP455106ENB
CSB-BP455106ENB
CSB-MP455106ENB
CSB-EP455106ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP455107ENB
CSB-EP455107ENB
CSB-BP455107ENB
CSB-MP455107ENB
CSB-EP455107ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Flagellar transcriptional regulator FlhD (flhD) CSB-YP455108ENB
CSB-EP455108ENB
CSB-BP455108ENB
CSB-MP455108ENB
CSB-EP455108ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Inosose dehydratase (iolE) CSB-YP455109ENB
CSB-EP455109ENB
CSB-BP455109ENB
CSB-MP455109ENB
CSB-EP455109ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Putative phosphotransferase ETA_18250 (ETA_18250) CSB-YP455110ENB
CSB-EP455110ENB
CSB-BP455110ENB
CSB-MP455110ENB
CSB-EP455110ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Protein slyX (slyX) CSB-YP455111ENB
CSB-EP455111ENB
CSB-BP455111ENB
CSB-MP455111ENB
CSB-EP455111ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 50S ribosomal protein L24 (rplX) CSB-YP455112ENB
CSB-EP455112ENB
CSB-BP455112ENB
CSB-MP455112ENB
CSB-EP455112ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 30S ribosomal protein S3 (rpsC) CSB-YP455113ENB
CSB-EP455113ENB
CSB-BP455113ENB
CSB-MP455113ENB
CSB-EP455113ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 50S ribosomal protein L17 (rplQ) CSB-YP455114ENB
CSB-EP455114ENB
CSB-BP455114ENB
CSB-MP455114ENB
CSB-EP455114ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 30S ribosomal protein S13 (rpsM) CSB-YP455115ENB
CSB-EP455115ENB
CSB-BP455115ENB
CSB-MP455115ENB
CSB-EP455115ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 50S ribosomal protein L6 (rplF) CSB-YP455116ENB
CSB-EP455116ENB
CSB-BP455116ENB
CSB-MP455116ENB
CSB-EP455116ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Large-conductance mechanosensitive channel (mscL), partial CSB-YP455117ENB1
CSB-EP455117ENB1
CSB-BP455117ENB1
CSB-MP455117ENB1
CSB-EP455117ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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