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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP453517RLV
CSB-EP453517RLV
CSB-BP453517RLV
CSB-MP453517RLV
CSB-EP453517RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Urease accessory protein UreG (ureG) CSB-YP453518RLV
CSB-EP453518RLV
CSB-BP453518RLV
CSB-MP453518RLV
CSB-EP453518RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Homoserine kinase (thrB) CSB-YP453519RLV
CSB-EP453519RLV
CSB-BP453519RLV
CSB-MP453519RLV
CSB-EP453519RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L27 (rpmA) CSB-YP453520RLV
CSB-EP453520RLV
CSB-BP453520RLV
CSB-MP453520RLV
CSB-EP453520RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP453521RLV
CSB-EP453521RLV
CSB-BP453521RLV
CSB-MP453521RLV
CSB-EP453521RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP453522RLV
CSB-EP453522RLV
CSB-BP453522RLV
CSB-MP453522RLV
CSB-EP453522RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L25 (rplY) CSB-YP453523RLV
CSB-EP453523RLV
CSB-BP453523RLV
CSB-MP453523RLV
CSB-EP453523RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA (guanine-N (1)-)-methyltransferase CSB-YP453524RLV
CSB-EP453524RLV
CSB-BP453524RLV
CSB-MP453524RLV
CSB-EP453524RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP453525RLV
CSB-EP453525RLV
CSB-BP453525RLV
CSB-MP453525RLV
CSB-EP453525RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0753 protein Rpal_3405 (Rpal_3405), partial CSB-YP453526RLV
CSB-EP453526RLV
CSB-BP453526RLV
CSB-MP453526RLV
CSB-EP453526RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Pantothenate kinase (coaA) CSB-YP453527RLV
CSB-EP453527RLV
CSB-BP453527RLV
CSB-MP453527RLV
CSB-EP453527RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP453528RLV
CSB-EP453528RLV
CSB-BP453528RLV
CSB-MP453528RLV
CSB-EP453528RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP453529RLV1
CSB-EP453529RLV1
CSB-BP453529RLV1
CSB-MP453529RLV1
CSB-EP453529RLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP453530RLV
CSB-EP453530RLV
CSB-BP453530RLV
CSB-MP453530RLV
CSB-EP453530RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP453531RLV
CSB-EP453531RLV
CSB-BP453531RLV
CSB-MP453531RLV
CSB-EP453531RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 3-dehydroquinate synthase (aroB) CSB-YP453532RLV
CSB-EP453532RLV
CSB-BP453532RLV
CSB-MP453532RLV
CSB-EP453532RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Homogentisate 1,2-dioxygenase (hmgA) CSB-YP453533RLV
CSB-EP453533RLV
CSB-BP453533RLV
CSB-MP453533RLV
CSB-EP453533RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP453534RLV
CSB-EP453534RLV
CSB-BP453534RLV
CSB-MP453534RLV
CSB-EP453534RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L30 (rpmD) CSB-YP453535RLV
CSB-EP453535RLV
CSB-BP453535RLV
CSB-MP453535RLV
CSB-EP453535RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L24 (rplX) CSB-YP453536RLV
CSB-EP453536RLV
CSB-BP453536RLV
CSB-MP453536RLV
CSB-EP453536RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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