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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella abortus Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP454698BWR
CSB-EP454698BWR
CSB-BP454698BWR
CSB-MP454698BWR
CSB-EP454698BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ribosomal protein L11 methyltransferase (prmA) CSB-YP454699BWR
CSB-EP454699BWR
CSB-BP454699BWR
CSB-MP454699BWR
CSB-EP454699BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Carbamoyl-phosphate synthase small chain (carA) CSB-YP454700BWR
CSB-EP454700BWR
CSB-BP454700BWR
CSB-MP454700BWR
CSB-EP454700BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus tRNA (Ile)-lysidine synthase CSB-YP454701BWR
CSB-EP454701BWR
CSB-BP454701BWR
CSB-MP454701BWR
CSB-EP454701BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP454702BWR
CSB-EP454702BWR
CSB-BP454702BWR
CSB-MP454702BWR
CSB-EP454702BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 4-hydroxyproline epimerase (BAbS19_I16820) CSB-YP454703BWR
CSB-EP454703BWR
CSB-BP454703BWR
CSB-MP454703BWR
CSB-EP454703BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP454704BWR
CSB-EP454704BWR
CSB-BP454704BWR
CSB-MP454704BWR
CSB-EP454704BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP454705BWR
CSB-EP454705BWR
CSB-BP454705BWR
CSB-MP454705BWR
CSB-EP454705BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus GTPase obg (obg) CSB-YP454706BWR
CSB-EP454706BWR
CSB-BP454706BWR
CSB-MP454706BWR
CSB-EP454706BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP454707BWR
CSB-EP454707BWR
CSB-BP454707BWR
CSB-MP454707BWR
CSB-EP454707BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 2-oxoglutarate dehydrogenase E1 component (sucA), partial CSB-YP454708BWR
CSB-EP454708BWR
CSB-BP454708BWR
CSB-MP454708BWR
CSB-EP454708BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ribonuclease PH (rph) CSB-YP454709BWR
CSB-EP454709BWR
CSB-BP454709BWR
CSB-MP454709BWR
CSB-EP454709BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP454710BWR
CSB-EP454710BWR
CSB-BP454710BWR
CSB-MP454710BWR
CSB-EP454710BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Shikimate dehydrogenase (aroE) CSB-YP454711BWR
CSB-EP454711BWR
CSB-BP454711BWR
CSB-MP454711BWR
CSB-EP454711BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Tryptophan synthase alpha chain (trpA) CSB-YP454712BWR
CSB-EP454712BWR
CSB-BP454712BWR
CSB-MP454712BWR
CSB-EP454712BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP454713BWR
CSB-EP454713BWR
CSB-BP454713BWR
CSB-MP454713BWR
CSB-EP454713BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP454714BWR
CSB-EP454714BWR
CSB-BP454714BWR
CSB-MP454714BWR
CSB-EP454714BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus UPF0434 protein BAbS19_II03260 (BAbS19_II03260) CSB-YP454715BWR
CSB-EP454715BWR
CSB-BP454715BWR
CSB-MP454715BWR
CSB-EP454715BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Blue-light-activated histidine kinase (BAbS19_II06090) CSB-YP454716BWR
CSB-EP454716BWR
CSB-BP454716BWR
CSB-MP454716BWR
CSB-EP454716BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP454717BWR
CSB-EP454717BWR
CSB-BP454717BWR
CSB-MP454717BWR
CSB-EP454717BWR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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