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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila grimshawi Enhancer of yellow 2 transcription factor (e (y)2) CSB-YP453798DLL
CSB-EP453798DLL
CSB-BP453798DLL
CSB-MP453798DLL
CSB-EP453798DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi AT-rich binding protein (ATbp) CSB-YP453799DLL
CSB-EP453799DLL
CSB-BP453799DLL
CSB-MP453799DLL
CSB-EP453799DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Mesencephalic astrocyte-derived neurotrophic factor homolog (Manf) CSB-YP453800DLL
CSB-EP453800DLL
CSB-BP453800DLL
CSB-MP453800DLL
CSB-EP453800DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Leucine-rich repeat protein soc-2 homolog (Sur-8), partial CSB-YP453801DLL
CSB-EP453801DLL
CSB-BP453801DLL
CSB-MP453801DLL
CSB-EP453801DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Ubiquitin carboxyl-terminal hydrolase calypso (calypso) CSB-YP453802DLL
CSB-EP453802DLL
CSB-BP453802DLL
CSB-MP453802DLL
CSB-EP453802DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi cGMP-specific 3',5'-cyclic phosphodiesterase (Pde6), partial CSB-YP453803DLL
CSB-EP453803DLL
CSB-BP453803DLL
CSB-MP453803DLL
CSB-EP453803DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Eukaryotic translation initiation factor 3 subunit G-2 (eIF3-S4-2) CSB-YP453804DLY
CSB-EP453804DLY
CSB-BP453804DLY
CSB-MP453804DLY
CSB-EP453804DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Adenylosuccinate synthetase (GI22153) CSB-YP453805DLY
CSB-EP453805DLY
CSB-BP453805DLY
CSB-MP453805DLY
CSB-EP453805DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Sensory neuron membrane protein 1 (Snmp1), partial CSB-YP453806DLY
CSB-EP453806DLY
CSB-BP453806DLY
CSB-MP453806DLY
CSB-EP453806DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Eukaryotic translation initiation factor 3 subunit F-1 (eIF3-S5-1) CSB-YP453807DLY
CSB-EP453807DLY
CSB-BP453807DLY
CSB-MP453807DLY
CSB-EP453807DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Protein cereblon homolog (GI23135), partial CSB-YP453808DLY
CSB-EP453808DLY
CSB-BP453808DLY
CSB-MP453808DLY
CSB-EP453808DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Ribosome biogenesis protein BOP1 homolog (GI19116), partial CSB-YP453809DLY
CSB-EP453809DLY
CSB-BP453809DLY
CSB-MP453809DLY
CSB-EP453809DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Lipoyl synthase, mitochondrial (Las), partial CSB-YP453810DLY
CSB-EP453810DLY
CSB-BP453810DLY
CSB-MP453810DLY
CSB-EP453810DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Enhancer of yellow 2 transcription factor (e (y)2) CSB-YP453811DLY
CSB-EP453811DLY
CSB-BP453811DLY
CSB-MP453811DLY
CSB-EP453811DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Serine/threonine-protein phosphatase Pgam5, mitochondrial (Pgam5) CSB-YP453812DLY
CSB-EP453812DLY
CSB-BP453812DLY
CSB-MP453812DLY
CSB-EP453812DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Sodium-dependent nutrient amino acid transporter 1 (NAAT1), partial CSB-YP453813DLY
CSB-EP453813DLY
CSB-BP453813DLY
CSB-MP453813DLY
CSB-EP453813DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Protein KIAA0664 homolog (GJ21424), partial CSB-YP453814DMP
CSB-EP453814DMP
CSB-BP453814DMP
CSB-MP453814DMP
CSB-EP453814DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Integrator complex subunit 3 homolog (IntS3), partial CSB-YP453815DMP
CSB-EP453815DMP
CSB-BP453815DMP
CSB-MP453815DMP
CSB-EP453815DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Eukaryotic translation initiation factor 3 subunit G-2 (eIF3-S4-2) CSB-YP453816DMP
CSB-EP453816DMP
CSB-BP453816DMP
CSB-MP453816DMP
CSB-EP453816DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Protein asunder (asun), partial CSB-YP453817DMP
CSB-EP453817DMP
CSB-BP453817DMP
CSB-MP453817DMP
CSB-EP453817DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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