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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bifidobacterium longum Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP455198BTA
CSB-EP455198BTA
CSB-BP455198BTA
CSB-MP455198BTA
CSB-EP455198BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Elongation factor 4 (lepA), partial CSB-YP455199BTA
CSB-EP455199BTA
CSB-BP455199BTA
CSB-MP455199BTA
CSB-EP455199BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Putative Holliday junction resolvase (BLD_0615) CSB-YP455200BTA
CSB-EP455200BTA
CSB-BP455200BTA
CSB-MP455200BTA
CSB-EP455200BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP455201BTA
CSB-EP455201BTA
CSB-BP455201BTA
CSB-MP455201BTA
CSB-EP455201BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP455202BTA
CSB-EP455202BTA
CSB-BP455202BTA
CSB-MP455202BTA
CSB-EP455202BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP455203BTA
CSB-EP455203BTA
CSB-BP455203BTA
CSB-MP455203BTA
CSB-EP455203BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum L-arabinose isomerase (araA), partial CSB-YP455204BTA
CSB-EP455204BTA
CSB-BP455204BTA
CSB-MP455204BTA
CSB-EP455204BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum D-alanine--D-alanine ligase (ddl) CSB-YP455205BTA
CSB-EP455205BTA
CSB-BP455205BTA
CSB-MP455205BTA
CSB-EP455205BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum ATP synthase subunit c (atpE), partial CSB-YP455206BTA1
CSB-EP455206BTA1
CSB-BP455206BTA1
CSB-MP455206BTA1
CSB-EP455206BTA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L9 (rplI) CSB-YP455207BTA
CSB-EP455207BTA
CSB-BP455207BTA
CSB-MP455207BTA
CSB-EP455207BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum Putative membrane protein insertion efficiency factor (Minf_0925) CSB-YP455208MSV
CSB-EP455208MSV
CSB-BP455208MSV
CSB-MP455208MSV
CSB-EP455208MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP455209MSV
CSB-EP455209MSV
CSB-BP455209MSV
CSB-MP455209MSV
CSB-EP455209MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP455210MSV
CSB-EP455210MSV
CSB-BP455210MSV
CSB-MP455210MSV
CSB-EP455210MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP455211MSV
CSB-EP455211MSV
CSB-BP455211MSV
CSB-MP455211MSV
CSB-EP455211MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum UPF0278 protein Minf_0007 (Minf_0007) CSB-YP455212MSV
CSB-EP455212MSV
CSB-BP455212MSV
CSB-MP455212MSV
CSB-EP455212MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum Aspartate--tRNA ligase (aspS), partial CSB-YP455213MSV
CSB-EP455213MSV
CSB-BP455213MSV
CSB-MP455213MSV
CSB-EP455213MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP455214MSV
CSB-EP455214MSV
CSB-BP455214MSV
CSB-MP455214MSV
CSB-EP455214MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP455215MSV1
CSB-EP455215MSV1
CSB-BP455215MSV1
CSB-MP455215MSV1
CSB-EP455215MSV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum Catalase-peroxidase (katG), partial CSB-YP455216MSV
CSB-EP455216MSV
CSB-BP455216MSV
CSB-MP455216MSV
CSB-EP455216MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum 30S ribosomal protein S3 (rpsC) CSB-YP455217MSV
CSB-EP455217MSV
CSB-BP455217MSV
CSB-MP455217MSV
CSB-EP455217MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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