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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila sechellia Eukaryotic translation initiation factor 3 subunit D-1 (eIF-3p66), partial CSB-YP453778DMG
CSB-EP453778DMG
CSB-BP453778DMG
CSB-MP453778DMG
CSB-EP453778DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Microtubule-associated protein Jupiter (Jupiter) CSB-YP453779DMG
CSB-EP453779DMG
CSB-BP453779DMG
CSB-MP453779DMG
CSB-EP453779DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Ras-like protein 1 (Ras85D) CSB-YP453780DMG
CSB-EP453780DMG
CSB-BP453780DMG
CSB-MP453780DMG
CSB-EP453780DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia WD repeat-containing protein 48 homolog (GM20456), partial CSB-YP453781DMG
CSB-EP453781DMG
CSB-BP453781DMG
CSB-MP453781DMG
CSB-EP453781DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Tyrosine-protein kinase-like otk (otk), partial CSB-YP453782DMG
CSB-EP453782DMG
CSB-BP453782DMG
CSB-MP453782DMG
CSB-EP453782DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Succinate dehydrogenase assembly factor 2-A, mitochondrial (GM20711), partial CSB-YP453783DMG
CSB-EP453783DMG
CSB-BP453783DMG
CSB-MP453783DMG
CSB-EP453783DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein alan shepard (shep), partial CSB-YP453784DMG
CSB-EP453784DMG
CSB-BP453784DMG
CSB-MP453784DMG
CSB-EP453784DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Glutamyl-tRNA (Gln) amidotransferase subunit C, mitochondrial, partial CSB-YP453785DMG
CSB-EP453785DMG
CSB-BP453785DMG
CSB-MP453785DMG
CSB-EP453785DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia tRNA (guanine-N (7)-)-methyltransferase CSB-YP453786DMG
CSB-EP453786DMG
CSB-BP453786DMG
CSB-MP453786DMG
CSB-EP453786DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Eukaryotic translation initiation factor 3 subunit F-2 (eIF3-S5-2) CSB-YP453787DMG
CSB-EP453787DMG
CSB-BP453787DMG
CSB-MP453787DMG
CSB-EP453787DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Protein Turandot C (TotC) CSB-YP453788DMG
CSB-EP453788DMG
CSB-BP453788DMG
CSB-MP453788DMG
CSB-EP453788DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia DM7 family protein GM11956 (GM11956), partial CSB-YP453789DMG
CSB-EP453789DMG
CSB-BP453789DMG
CSB-MP453789DMG
CSB-EP453789DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Integrator complex subunit 3 homolog (IntS3), partial CSB-YP453790DMG
CSB-EP453790DMG
CSB-BP453790DMG
CSB-MP453790DMG
CSB-EP453790DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Protein Turandot X (TotX) CSB-YP453791DMR
CSB-EP453791DMR
CSB-BP453791DMR
CSB-MP453791DMR
CSB-EP453791DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Calcium channel flower (flower), partial CSB-YP453792DLL1
CSB-EP453792DLL1
CSB-BP453792DLL1
CSB-MP453792DLL1
CSB-EP453792DLL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Succinate dehydrogenase assembly factor 2, mitochondrial (GH20828), partial CSB-YP453793DLL
CSB-EP453793DLL
CSB-BP453793DLL
CSB-MP453793DLL
CSB-EP453793DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Latrophilin Cirl (Cirl), partial CSB-YP453794DLL
CSB-EP453794DLL
CSB-BP453794DLL
CSB-MP453794DLL
CSB-EP453794DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Eukaryotic translation initiation factor 3 subunit F-2 (eIF3-S5-2) CSB-YP453795DLL
CSB-EP453795DLL
CSB-BP453795DLL
CSB-MP453795DLL
CSB-EP453795DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Transmembrane emp24 domain-containing protein eca (eca), partial CSB-YP453796DLL
CSB-EP453796DLL
CSB-BP453796DLL
CSB-MP453796DLL
CSB-EP453796DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Protein asunder (asun), partial CSB-YP453797DLL
CSB-EP453797DLL
CSB-BP453797DLL
CSB-MP453797DLL
CSB-EP453797DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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