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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Proteus mirabilis Flagellar basal body rod protein FlgB (flgB) CSB-YP453738EYZ
CSB-EP453738EYZ
CSB-BP453738EYZ
CSB-MP453738EYZ
CSB-EP453738EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L1 (rplA) CSB-YP453739EYZ
CSB-EP453739EYZ
CSB-BP453739EYZ
CSB-MP453739EYZ
CSB-EP453739EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0270 protein PMI2817 (PMI2817) CSB-YP453740EYZ
CSB-EP453740EYZ
CSB-BP453740EYZ
CSB-MP453740EYZ
CSB-EP453740EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP453741EYZ
CSB-EP453741EYZ
CSB-BP453741EYZ
CSB-MP453741EYZ
CSB-EP453741EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Fe/S biogenesis protein NfuA (nfuA) CSB-YP453742EYZ
CSB-EP453742EYZ
CSB-BP453742EYZ
CSB-MP453742EYZ
CSB-EP453742EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Histidine--tRNA ligase (hisS) CSB-YP453743EYZ
CSB-EP453743EYZ
CSB-BP453743EYZ
CSB-MP453743EYZ
CSB-EP453743EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP453744EYZ
CSB-EP453744EYZ
CSB-BP453744EYZ
CSB-MP453744EYZ
CSB-EP453744EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Elongation factor 4 (lepA), partial CSB-YP453745EYZ
CSB-EP453745EYZ
CSB-BP453745EYZ
CSB-MP453745EYZ
CSB-EP453745EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Protein FdhD homolog (fdhD) CSB-YP453746EYZ
CSB-EP453746EYZ
CSB-BP453746EYZ
CSB-MP453746EYZ
CSB-EP453746EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP453747EYZ
CSB-EP453747EYZ
CSB-BP453747EYZ
CSB-MP453747EYZ
CSB-EP453747EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP453748EYZ
CSB-EP453748EYZ
CSB-BP453748EYZ
CSB-MP453748EYZ
CSB-EP453748EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Regulator of ribonuclease activity A (rraA) CSB-YP453749EYZ
CSB-EP453749EYZ
CSB-BP453749EYZ
CSB-MP453749EYZ
CSB-EP453749EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP453750EYZ
CSB-EP453750EYZ
CSB-BP453750EYZ
CSB-MP453750EYZ
CSB-EP453750EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis S-adenosylmethionine synthase (metK) CSB-YP453751EYZ
CSB-EP453751EYZ
CSB-BP453751EYZ
CSB-MP453751EYZ
CSB-EP453751EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Glutamate racemase (murI) CSB-YP453752EYZ
CSB-EP453752EYZ
CSB-BP453752EYZ
CSB-MP453752EYZ
CSB-EP453752EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 30S ribosomal protein S19 (rpsS) CSB-YP453753EYZ
CSB-EP453753EYZ
CSB-BP453753EYZ
CSB-MP453753EYZ
CSB-EP453753EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L14 (rplN) CSB-YP453754EYZ
CSB-EP453754EYZ
CSB-BP453754EYZ
CSB-MP453754EYZ
CSB-EP453754EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L36 (rpmJ) CSB-YP453755EYZ
CSB-EP453755EYZ
CSB-BP453755EYZ
CSB-MP453755EYZ
CSB-EP453755EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Shikimate dehydrogenase (aroE) CSB-YP453756EYZ
CSB-EP453756EYZ
CSB-BP453756EYZ
CSB-MP453756EYZ
CSB-EP453756EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis DNA mismatch repair protein mutL (mutL), partial CSB-YP453757EYZ
CSB-EP453757EYZ
CSB-BP453757EYZ
CSB-MP453757EYZ
CSB-EP453757EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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