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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium marinum UPF0678 fatty acid-binding protein-like protein MMAR_4871 (MMAR_4871) CSB-YP454318MVS
CSB-EP454318MVS
CSB-BP454318MVS
CSB-MP454318MVS
CSB-EP454318MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP454319MVS
CSB-EP454319MVS
CSB-BP454319MVS
CSB-MP454319MVS
CSB-EP454319MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UPF0232 protein MMAR_0004 (MMAR_0004) CSB-YP454320MVS
CSB-EP454320MVS
CSB-BP454320MVS
CSB-MP454320MVS
CSB-EP454320MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UPF0233 membrane protein MMAR_0013 (MMAR_0013), partial CSB-YP454321MVS1
CSB-EP454321MVS1
CSB-BP454321MVS1
CSB-MP454321MVS1
CSB-EP454321MVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 3-isopropylmalate dehydrogenase (leuB) CSB-YP454322MVS
CSB-EP454322MVS
CSB-BP454322MVS
CSB-MP454322MVS
CSB-EP454322MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Long-chain-fatty-acid--AMP ligase FadD29 (fadD29), partial CSB-YP454323MVS
CSB-EP454323MVS
CSB-BP454323MVS
CSB-MP454323MVS
CSB-EP454323MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP454324MVS
CSB-EP454324MVS
CSB-BP454324MVS
CSB-MP454324MVS
CSB-EP454324MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UPF0102 protein MMAR_1812 (MMAR_1812) CSB-YP454325MVS
CSB-EP454325MVS
CSB-BP454325MVS
CSB-MP454325MVS
CSB-EP454325MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S18 2 (rpsR2) CSB-YP454326MVS
CSB-EP454326MVS
CSB-BP454326MVS
CSB-MP454326MVS
CSB-EP454326MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S15 (rpsO) CSB-YP454327MVS
CSB-EP454327MVS
CSB-BP454327MVS
CSB-MP454327MVS
CSB-EP454327MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum tRNA dimethylallyltransferase 2 (miaA2) CSB-YP454328MVS
CSB-EP454328MVS
CSB-BP454328MVS
CSB-MP454328MVS
CSB-EP454328MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Maltokinase (pep2) CSB-YP454329MVS
CSB-EP454329MVS
CSB-BP454329MVS
CSB-MP454329MVS
CSB-EP454329MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Threonine--tRNA ligase (thrS), partial CSB-YP454330MVS
CSB-EP454330MVS
CSB-BP454330MVS
CSB-MP454330MVS
CSB-EP454330MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Glutamate 5-kinase (proB) CSB-YP454331MVS
CSB-EP454331MVS
CSB-BP454331MVS
CSB-MP454331MVS
CSB-EP454331MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Histidine--tRNA ligase (hisS) CSB-YP454332MVS
CSB-EP454332MVS
CSB-BP454332MVS
CSB-MP454332MVS
CSB-EP454332MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Aspartate carbamoyltransferase (pyrB) CSB-YP454333MVS
CSB-EP454333MVS
CSB-BP454333MVS
CSB-MP454333MVS
CSB-EP454333MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Trigger factor (tig) CSB-YP454334MVS
CSB-EP454334MVS
CSB-BP454334MVS
CSB-MP454334MVS
CSB-EP454334MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum S-adenosylmethionine synthase (metK) CSB-YP454335MVS
CSB-EP454335MVS
CSB-BP454335MVS
CSB-MP454335MVS
CSB-EP454335MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UPF0042 nucleotide-binding protein MMAR_2228 (MMAR_2228) CSB-YP454336MVS
CSB-EP454336MVS
CSB-BP454336MVS
CSB-MP454336MVS
CSB-EP454336MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP454337MVS
CSB-EP454337MVS
CSB-BP454337MVS
CSB-MP454337MVS
CSB-EP454337MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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