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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP455319AXI
CSB-EP455319AXI
CSB-BP455319AXI
CSB-MP455319AXI
CSB-EP455319AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP455320AXI
CSB-EP455320AXI
CSB-BP455320AXI
CSB-MP455320AXI
CSB-EP455320AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L10 (rplJ) CSB-YP455321AXI
CSB-EP455321AXI
CSB-BP455321AXI
CSB-MP455321AXI
CSB-EP455321AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L5 (rplE) CSB-YP455322AXI
CSB-EP455322AXI
CSB-BP455322AXI
CSB-MP455322AXI
CSB-EP455322AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L18 (rplR) CSB-YP455323AXI
CSB-EP455323AXI
CSB-BP455323AXI
CSB-MP455323AXI
CSB-EP455323AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Chaperone protein TorD (torD) CSB-YP455324AXI
CSB-EP455324AXI
CSB-BP455324AXI
CSB-MP455324AXI
CSB-EP455324AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP455325AXI
CSB-EP455325AXI
CSB-BP455325AXI
CSB-MP455325AXI
CSB-EP455325AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP455326AXI
CSB-EP455326AXI
CSB-BP455326AXI
CSB-MP455326AXI
CSB-EP455326AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 ATP phosphoribosyltransferase (hisG) CSB-YP455327AXI
CSB-EP455327AXI
CSB-BP455327AXI
CSB-MP455327AXI
CSB-EP455327AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP455328AXI
CSB-EP455328AXI
CSB-BP455328AXI
CSB-MP455328AXI
CSB-EP455328AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Isoleucine--tRNA ligase (ileS), partial CSB-YP455329AXI
CSB-EP455329AXI
CSB-BP455329AXI
CSB-MP455329AXI
CSB-EP455329AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP455330AXI
CSB-EP455330AXI
CSB-BP455330AXI
CSB-MP455330AXI
CSB-EP455330AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Electron transport complex protein RnfD (rnfD), partial CSB-YP455331AXI
CSB-EP455331AXI
CSB-BP455331AXI
CSB-MP455331AXI
CSB-EP455331AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L35 (rpmI) CSB-YP455332AXI
CSB-EP455332AXI
CSB-BP455332AXI
CSB-MP455332AXI
CSB-EP455332AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP455333AXI
CSB-EP455333AXI
CSB-BP455333AXI
CSB-MP455333AXI
CSB-EP455333AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Probable endonuclease 4 (nfo) CSB-YP455334AXI
CSB-EP455334AXI
CSB-BP455334AXI
CSB-MP455334AXI
CSB-EP455334AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP455335AXI
CSB-EP455335AXI
CSB-BP455335AXI
CSB-MP455335AXI
CSB-EP455335AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 GTPase Der (der), partial CSB-YP455336AXI
CSB-EP455336AXI
CSB-BP455336AXI
CSB-MP455336AXI
CSB-EP455336AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Asparagine--tRNA ligase (asnS) CSB-YP455337AXI
CSB-EP455337AXI
CSB-BP455337AXI
CSB-MP455337AXI
CSB-EP455337AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Outer-membrane lipoprotein lolB (lolB) CSB-YP455338AXI
CSB-EP455338AXI
CSB-BP455338AXI
CSB-MP455338AXI
CSB-EP455338AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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