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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium marinum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP454338MVS
CSB-EP454338MVS
CSB-BP454338MVS
CSB-MP454338MVS
CSB-EP454338MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP454339MVS
CSB-EP454339MVS
CSB-BP454339MVS
CSB-MP454339MVS
CSB-EP454339MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum ATP synthase gamma chain (atpG) CSB-YP454340MVS
CSB-EP454340MVS
CSB-BP454340MVS
CSB-MP454340MVS
CSB-EP454340MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX (lysX), partial CSB-YP454341MVS
CSB-EP454341MVS
CSB-BP454341MVS
CSB-MP454341MVS
CSB-EP454341MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP454342MVS
CSB-EP454342MVS
CSB-BP454342MVS
CSB-MP454342MVS
CSB-EP454342MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_0955 (MMAR_0955) CSB-YP454343MVS
CSB-EP454343MVS
CSB-BP454343MVS
CSB-MP454343MVS
CSB-EP454343MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S19 (rpsS) CSB-YP454344MVS
CSB-EP454344MVS
CSB-BP454344MVS
CSB-MP454344MVS
CSB-EP454344MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S17 (rpsQ) CSB-YP454345MVS
CSB-EP454345MVS
CSB-BP454345MVS
CSB-MP454345MVS
CSB-EP454345MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP454346MVS
CSB-EP454346MVS
CSB-BP454346MVS
CSB-MP454346MVS
CSB-EP454346MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii UPF0042 nucleotide-binding protein ACICU_00591 (ACICU_00591) CSB-YP454347AWO
CSB-EP454347AWO
CSB-BP454347AWO
CSB-MP454347AWO
CSB-EP454347AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L35 (rpmI) CSB-YP454348AWO
CSB-EP454348AWO
CSB-BP454348AWO
CSB-MP454348AWO
CSB-EP454348AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP454349AWO
CSB-EP454349AWO
CSB-BP454349AWO
CSB-MP454349AWO
CSB-EP454349AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L9 (rplI) CSB-YP454350AWO
CSB-EP454350AWO
CSB-BP454350AWO
CSB-MP454350AWO
CSB-EP454350AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L32 (rpmF) CSB-YP454351AWO
CSB-EP454351AWO
CSB-BP454351AWO
CSB-MP454351AWO
CSB-EP454351AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable septum site-determining protein MinC (minC) CSB-YP454352AWO
CSB-EP454352AWO
CSB-BP454352AWO
CSB-MP454352AWO
CSB-EP454352AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Thiazole synthase (thiG) CSB-YP454353AWO
CSB-EP454353AWO
CSB-BP454353AWO
CSB-MP454353AWO
CSB-EP454353AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP454354AWO
CSB-EP454354AWO
CSB-BP454354AWO
CSB-MP454354AWO
CSB-EP454354AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Betaine aldehyde dehydrogenase (betB) CSB-YP454355AWO
CSB-EP454355AWO
CSB-BP454355AWO
CSB-MP454355AWO
CSB-EP454355AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L31 (rpmE) CSB-YP454356AWO
CSB-EP454356AWO
CSB-BP454356AWO
CSB-MP454356AWO
CSB-EP454356AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP454357AWO
CSB-EP454357AWO
CSB-BP454357AWO
CSB-MP454357AWO
CSB-EP454357AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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