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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP454918SYZ
CSB-EP454918SYZ
CSB-BP454918SYZ
CSB-MP454918SYZ
CSB-EP454918SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0133 protein ybaB (ybaB) CSB-YP454919SYZ
CSB-EP454919SYZ
CSB-BP454919SYZ
CSB-MP454919SYZ
CSB-EP454919SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Copper homeostasis protein CutC (cutC) CSB-YP454920SYZ
CSB-EP454920SYZ
CSB-BP454920SYZ
CSB-MP454920SYZ
CSB-EP454920SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein-export protein secB (secB) CSB-YP454921SYZ
CSB-EP454921SYZ
CSB-BP454921SYZ
CSB-MP454921SYZ
CSB-EP454921SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP454922RJI
CSB-EP454922RJI
CSB-BP454922RJI
CSB-MP454922RJI
CSB-EP454922RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 7-cyano-7-deazaguanine synthase (queC) CSB-YP454923RJI
CSB-EP454923RJI
CSB-BP454923RJI
CSB-MP454923RJI
CSB-EP454923RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Urease subunit beta (ureB) CSB-YP454924RJI
CSB-EP454924RJI
CSB-BP454924RJI
CSB-MP454924RJI
CSB-EP454924RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP454925RJI
CSB-EP454925RJI
CSB-BP454925RJI
CSB-MP454925RJI
CSB-EP454925RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Homoserine O-acetyltransferase (metX) CSB-YP454926RJI
CSB-EP454926RJI
CSB-BP454926RJI
CSB-MP454926RJI
CSB-EP454926RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Glutamine--tRNA ligase (glnS), partial CSB-YP454927RJI
CSB-EP454927RJI
CSB-BP454927RJI
CSB-MP454927RJI
CSB-EP454927RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP454928RJI
CSB-EP454928RJI
CSB-BP454928RJI
CSB-MP454928RJI
CSB-EP454928RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Peptide chain release factor 2 (prfB) CSB-YP454929RJI
CSB-EP454929RJI
CSB-BP454929RJI
CSB-MP454929RJI
CSB-EP454929RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP454930RJI
CSB-EP454930RJI
CSB-BP454930RJI
CSB-MP454930RJI
CSB-EP454930RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP454931RJI
CSB-EP454931RJI
CSB-BP454931RJI
CSB-MP454931RJI
CSB-EP454931RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Cysteine--tRNA ligase (cysS) CSB-YP454932RJI
CSB-EP454932RJI
CSB-BP454932RJI
CSB-MP454932RJI
CSB-EP454932RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP454933RJI
CSB-EP454933RJI
CSB-BP454933RJI
CSB-MP454933RJI
CSB-EP454933RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Outer-membrane lipoprotein carrier protein (lolA) CSB-YP454934RJI
CSB-EP454934RJI
CSB-BP454934RJI
CSB-MP454934RJI
CSB-EP454934RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L9 (rplI) CSB-YP454935RJI
CSB-EP454935RJI
CSB-BP454935RJI
CSB-MP454935RJI
CSB-EP454935RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP454936RJI
CSB-EP454936RJI
CSB-BP454936RJI
CSB-MP454936RJI
CSB-EP454936RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 8-amino-7-oxononanoate synthase (bioF) CSB-YP454937RJI
CSB-EP454937RJI
CSB-BP454937RJI
CSB-MP454937RJI
CSB-EP454937RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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