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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Arginine--tRNA ligase, cytoplasmic (RARS) CSB-YP019344HU
CSB-EP019344HU
CSB-BP019344HU
CSB-MP019344HU
CSB-EP019344HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Homeobox protein Hox-A9 (HOXA9) CSB-YP010658GU
CSB-EP010658GU
CSB-BP010658GU
CSB-MP010658GU
CSB-EP010658GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human H (+)/Cl (-) exchange transporter 4 (CLCN4), partial CSB-YP005483HU
CSB-EP005483HU
CSB-BP005483HU
CSB-MP005483HU
CSB-EP005483HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Chloride channel protein ClC-Ka (CLCNKA), partial CSB-YP005487HU
CSB-EP005487HU
CSB-BP005487HU
CSB-MP005487HU
CSB-EP005487HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ribosomal protein S6 kinase alpha-3 (RPS6KA3) CSB-YP020466HU
CSB-EP020466HU
CSB-BP020466HU
CSB-MP020466HU
CSB-EP020466HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse AF4/FMR2 family member 3 (Aff3), partial CSB-YP001414MO
CSB-EP001414MO
CSB-BP001414MO
CSB-MP001414MO
CSB-EP001414MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Lumican (LUM) CSB-YP013234CH
CSB-EP013234CH
CSB-BP013234CH
CSB-MP013234CH
CSB-EP013234CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cyclin-F (Ccnf) CSB-YP004819MO
CSB-EP004819MO
CSB-BP004819MO
CSB-MP004819MO
CSB-EP004819MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Paired box protein Pax-8 (Pax8) CSB-YP017494RA
CSB-EP017494RA
CSB-BP017494RA
CSB-MP017494RA
CSB-EP017494RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Squalene monooxygenase (Sqle), partial CSB-YP022645MO1
CSB-EP022645MO1
CSB-BP022645MO1
CSB-MP022645MO1
CSB-EP022645MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Calmegin (Clgn), partial CSB-YP005544MO1
CSB-EP005544MO1
CSB-BP005544MO1
CSB-MP005544MO1
CSB-EP005544MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Guanine nucleotide-binding protein subunit alpha-11 (GNA11) CSB-YP009583DO
CSB-EP009583DO
CSB-BP009583DO
CSB-MP009583DO
CSB-EP009583DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 6-phosphogluconate dehydrogenase, decarboxylating (PGD) CSB-YP017850HU
CSB-EP017850HU
CSB-BP017850HU
CSB-MP017850HU
CSB-EP017850HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Importin subunit alpha-1 (KPNA2) CSB-YP012484HU
CSB-BP012484HU
CSB-EP012484HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 10 (Usp10) CSB-YP025696MO
CSB-EP025696MO
CSB-BP025696MO
CSB-MP025696MO
CSB-EP025696MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Acyl carrier protein, mitochondrial (NDUFAB1) CSB-YP015636BO
CSB-EP015636BO
CSB-BP015636BO
CSB-MP015636BO
CSB-EP015636BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Nuclear envelope pore membrane protein POM 121 (Pom121), partial CSB-YP018356RA
CSB-EP018356RA
CSB-BP018356RA
CSB-MP018356RA
CSB-EP018356RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Heterogeneous nuclear ribonucleoprotein F (HNRNPF) CSB-YP010608HU
CSB-EP010608HU
CSB-BP010608HU
CSB-MP010608HU
CSB-EP010608HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Kinesin-like protein KIF11 (KIF11), partial CSB-YP012307HU
CSB-EP012307HU
CSB-BP012307HU
CSB-MP012307HU
CSB-EP012307HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein 132 (ZNF132) CSB-YP026531HU
CSB-EP026531HU
CSB-BP026531HU
CSB-MP026531HU
CSB-EP026531HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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