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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 Protein FdhE (fdhE) CSB-YP456760SYZ
CSB-EP456760SYZ
CSB-BP456760SYZ
CSB-MP456760SYZ
CSB-EP456760SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Probable endonuclease 4 (nfo) CSB-YP456761SYZ
CSB-EP456761SYZ
CSB-BP456761SYZ
CSB-MP456761SYZ
CSB-EP456761SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP456762SYZ
CSB-EP456762SYZ
CSB-BP456762SYZ
CSB-MP456762SYZ
CSB-EP456762SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP456763SYZ
CSB-EP456763SYZ
CSB-BP456763SYZ
CSB-MP456763SYZ
CSB-EP456763SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0304 protein yfbU (yfbU) CSB-YP456764SYZ
CSB-EP456764SYZ
CSB-BP456764SYZ
CSB-MP456764SYZ
CSB-EP456764SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0115 protein yfcN (yfcN) CSB-YP456765SYZ
CSB-EP456765SYZ
CSB-BP456765SYZ
CSB-MP456765SYZ
CSB-EP456765SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L31 (rpmE) CSB-YP456766SYZ
CSB-EP456766SYZ
CSB-BP456766SYZ
CSB-MP456766SYZ
CSB-EP456766SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP456767SYZ
CSB-EP456767SYZ
CSB-BP456767SYZ
CSB-MP456767SYZ
CSB-EP456767SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Thiamine-phosphate synthase (thiE) CSB-YP456768SYZ
CSB-EP456768SYZ
CSB-BP456768SYZ
CSB-MP456768SYZ
CSB-EP456768SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP456769SYZ
CSB-EP456769SYZ
CSB-BP456769SYZ
CSB-MP456769SYZ
CSB-EP456769SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein mtfA (mtfA) CSB-YP456770SYZ
CSB-EP456770SYZ
CSB-BP456770SYZ
CSB-MP456770SYZ
CSB-EP456770SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glutamate--tRNA ligase (gltX) CSB-YP456771SYZ
CSB-EP456771SYZ
CSB-BP456771SYZ
CSB-MP456771SYZ
CSB-EP456771SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Cytochrome c-552 (nrfA) CSB-YP456772SYZ
CSB-EP456772SYZ
CSB-BP456772SYZ
CSB-MP456772SYZ
CSB-EP456772SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0370 protein ypfN (ypfN), partial CSB-YP456773SYZ
CSB-EP456773SYZ
CSB-BP456773SYZ
CSB-MP456773SYZ
CSB-EP456773SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Uracil phosphoribosyltransferase (upp) CSB-YP456774SYZ
CSB-EP456774SYZ
CSB-BP456774SYZ
CSB-MP456774SYZ
CSB-EP456774SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Co-chaperone protein hscB (hscB) CSB-YP456775SYZ
CSB-EP456775SYZ
CSB-BP456775SYZ
CSB-MP456775SYZ
CSB-EP456775SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 10 kDa chaperonin (groS) CSB-YP456776SYZ
CSB-EP456776SYZ
CSB-BP456776SYZ
CSB-MP456776SYZ
CSB-EP456776SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 HTH-type transcriptional repressor NsrR (nsrR) CSB-YP456777SYZ
CSB-EP456777SYZ
CSB-BP456777SYZ
CSB-MP456777SYZ
CSB-EP456777SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L9 (rplI) CSB-YP456778SYZ
CSB-EP456778SYZ
CSB-BP456778SYZ
CSB-MP456778SYZ
CSB-EP456778SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Multidrug resistance protein MdtC (mdtC), partial CSB-YP456779SYZ
CSB-EP456779SYZ
CSB-BP456779SYZ
CSB-MP456779SYZ
CSB-EP456779SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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