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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter lovleyi Glutamate--tRNA ligase (gltX) CSB-YP457120GFO
CSB-EP457120GFO
CSB-BP457120GFO
CSB-MP457120GFO
CSB-EP457120GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 30S ribosomal protein S20 (rpsT) CSB-YP457121GFO
CSB-EP457121GFO
CSB-BP457121GFO
CSB-MP457121GFO
CSB-EP457121GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L34 (rpmH) CSB-YP457122GFO
CSB-EP457122GFO
CSB-BP457122GFO
CSB-MP457122GFO
CSB-EP457122GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Lipid II flippase FtsW (ftsW), partial CSB-YP457123GFO
CSB-EP457123GFO
CSB-BP457123GFO
CSB-MP457123GFO
CSB-EP457123GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi UPF0042 nucleotide-binding protein Glov_2163 (Glov_2163) CSB-YP457124GFO
CSB-EP457124GFO
CSB-BP457124GFO
CSB-MP457124GFO
CSB-EP457124GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi UPF0102 protein Glov_2230 (Glov_2230) CSB-YP457125GFO
CSB-EP457125GFO
CSB-BP457125GFO
CSB-MP457125GFO
CSB-EP457125GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi tRNA dimethylallyltransferase 2 (miaA2) CSB-YP457126GFO
CSB-EP457126GFO
CSB-BP457126GFO
CSB-MP457126GFO
CSB-EP457126GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Bifunctional protein HldE (hldE) CSB-YP457127GFO
CSB-EP457127GFO
CSB-BP457127GFO
CSB-MP457127GFO
CSB-EP457127GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi tRNA dimethylallyltransferase 1 (miaA1) CSB-YP457128GFO
CSB-EP457128GFO
CSB-BP457128GFO
CSB-MP457128GFO
CSB-EP457128GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP457129GFO
CSB-EP457129GFO
CSB-BP457129GFO
CSB-MP457129GFO
CSB-EP457129GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Phosphoglucosamine mutase (glmM) CSB-YP457130GFO
CSB-EP457130GFO
CSB-BP457130GFO
CSB-MP457130GFO
CSB-EP457130GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi tRNA (cmo5U34)-methyltransferase CSB-YP457131GFO
CSB-EP457131GFO
CSB-BP457131GFO
CSB-MP457131GFO
CSB-EP457131GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Selenide, water dikinase (selD) CSB-YP457132GFO
CSB-EP457132GFO
CSB-BP457132GFO
CSB-MP457132GFO
CSB-EP457132GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L7/L12 (rplL) CSB-YP457133GFO
CSB-EP457133GFO
CSB-BP457133GFO
CSB-MP457133GFO
CSB-EP457133GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 30S ribosomal protein S12 (rpsL) CSB-YP457134GFO
CSB-EP457134GFO
CSB-BP457134GFO
CSB-MP457134GFO
CSB-EP457134GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L4 (rplD) CSB-YP457135GFO
CSB-EP457135GFO
CSB-BP457135GFO
CSB-MP457135GFO
CSB-EP457135GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L22 (rplV) CSB-YP457136GFO
CSB-EP457136GFO
CSB-BP457136GFO
CSB-MP457136GFO
CSB-EP457136GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Chaperone protein DnaK (dnaK), partial CSB-YP457137GFO
CSB-EP457137GFO
CSB-BP457137GFO
CSB-MP457137GFO
CSB-EP457137GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 50S ribosomal protein L36 (rpmJ) CSB-YP457138GFO
CSB-EP457138GFO
CSB-BP457138GFO
CSB-MP457138GFO
CSB-EP457138GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi DNA ligase (ligA), partial CSB-YP457139GFO
CSB-EP457139GFO
CSB-BP457139GFO
CSB-MP457139GFO
CSB-EP457139GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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