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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chlorobium limicola 7-cyano-7-deazaguanine synthase (queC) CSB-YP457160DSR
CSB-EP457160DSR
CSB-BP457160DSR
CSB-MP457160DSR
CSB-EP457160DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 30S ribosomal protein S21 (rpsU) CSB-YP457161DSR
CSB-EP457161DSR
CSB-BP457161DSR
CSB-MP457161DSR
CSB-EP457161DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Light-independent protochlorophyllide reductase subunit B (bchB), partial CSB-YP457162DSR
CSB-EP457162DSR
CSB-BP457162DSR
CSB-MP457162DSR
CSB-EP457162DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 50S ribosomal protein L30 (rpmD) CSB-YP457163DSR
CSB-EP457163DSR
CSB-BP457163DSR
CSB-MP457163DSR
CSB-EP457163DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 50S ribosomal protein L5 (rplE) CSB-YP457164DSR
CSB-EP457164DSR
CSB-BP457164DSR
CSB-MP457164DSR
CSB-EP457164DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 50S ribosomal protein L22 (rplV) CSB-YP457165DSR
CSB-EP457165DSR
CSB-BP457165DSR
CSB-MP457165DSR
CSB-EP457165DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP457166DSR
CSB-EP457166DSR
CSB-BP457166DSR
CSB-MP457166DSR
CSB-EP457166DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 30S ribosomal protein S7 (rpsG) CSB-YP457167DSR
CSB-EP457167DSR
CSB-BP457167DSR
CSB-MP457167DSR
CSB-EP457167DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola CinA-like protein (Clim_2329) CSB-YP457168DSR
CSB-EP457168DSR
CSB-BP457168DSR
CSB-MP457168DSR
CSB-EP457168DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP457169DSR
CSB-EP457169DSR
CSB-BP457169DSR
CSB-MP457169DSR
CSB-EP457169DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Biotin synthase (bioB) CSB-YP457170DSR
CSB-EP457170DSR
CSB-BP457170DSR
CSB-MP457170DSR
CSB-EP457170DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Recombination protein RecR (recR) CSB-YP457171DSR
CSB-EP457171DSR
CSB-BP457171DSR
CSB-MP457171DSR
CSB-EP457171DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Probable transaldolase (tal) CSB-YP457172DSR
CSB-EP457172DSR
CSB-BP457172DSR
CSB-MP457172DSR
CSB-EP457172DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP457173DSR
CSB-EP457173DSR
CSB-BP457173DSR
CSB-MP457173DSR
CSB-EP457173DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Phosphoglycerate kinase (pgk) CSB-YP457174DSR
CSB-EP457174DSR
CSB-BP457174DSR
CSB-MP457174DSR
CSB-EP457174DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides UPF0102 protein Cphamn1_0017 (Cphamn1_0017) CSB-YP457175DSS
CSB-EP457175DSS
CSB-BP457175DSS
CSB-MP457175DSS
CSB-EP457175DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L20 (rplT) CSB-YP457176DSS
CSB-EP457176DSS
CSB-BP457176DSS
CSB-MP457176DSS
CSB-EP457176DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP457177DSS
CSB-EP457177DSS
CSB-BP457177DSS
CSB-MP457177DSS
CSB-EP457177DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides ATP synthase gamma chain (atpG) CSB-YP457178DSS
CSB-EP457178DSS
CSB-BP457178DSS
CSB-MP457178DSS
CSB-EP457178DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Nitrogenase iron protein (nifH) CSB-YP457179DSS
CSB-EP457179DSS
CSB-BP457179DSS
CSB-MP457179DSS
CSB-EP457179DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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