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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus casei D-alanine--D-alanine ligase (ddl) CSB-YP457521LMJ
CSB-EP457521LMJ
CSB-BP457521LMJ
CSB-MP457521LMJ
CSB-EP457521LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 6-phospho-beta-galactosidase (lacG) CSB-YP457522LMJ
CSB-EP457522LMJ
CSB-BP457522LMJ
CSB-MP457522LMJ
CSB-EP457522LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Serine--tRNA ligase (serS) CSB-YP457523LMJ
CSB-EP457523LMJ
CSB-BP457523LMJ
CSB-MP457523LMJ
CSB-EP457523LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei UPF0246 protein LCABL_22600 (LCABL_22600) CSB-YP457524LMJ
CSB-EP457524LMJ
CSB-BP457524LMJ
CSB-MP457524LMJ
CSB-EP457524LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP457525LMJ
CSB-EP457525LMJ
CSB-BP457525LMJ
CSB-MP457525LMJ
CSB-EP457525LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Recombination protein RecR (recR) CSB-YP457526LMJ
CSB-EP457526LMJ
CSB-BP457526LMJ
CSB-MP457526LMJ
CSB-EP457526LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L13 (rplM) CSB-YP457527LMJ
CSB-EP457527LMJ
CSB-BP457527LMJ
CSB-MP457527LMJ
CSB-EP457527LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L14 (rplN) CSB-YP457528LMJ
CSB-EP457528LMJ
CSB-BP457528LMJ
CSB-MP457528LMJ
CSB-EP457528LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L23 (rplW) CSB-YP457529LMJ
CSB-EP457529LMJ
CSB-BP457529LMJ
CSB-MP457529LMJ
CSB-EP457529LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 30S ribosomal protein S12 (rpsL) CSB-YP457530LMJ
CSB-EP457530LMJ
CSB-BP457530LMJ
CSB-MP457530LMJ
CSB-EP457530LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP457531LMJ
CSB-EP457531LMJ
CSB-BP457531LMJ
CSB-MP457531LMJ
CSB-EP457531LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP457532LMJ
CSB-EP457532LMJ
CSB-BP457532LMJ
CSB-MP457532LMJ
CSB-EP457532LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei MutS2 protein (mutS2), partial CSB-YP457533LMJ
CSB-EP457533LMJ
CSB-BP457533LMJ
CSB-MP457533LMJ
CSB-EP457533LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei S-adenosylmethionine synthase (metK) CSB-YP457534LMJ
CSB-EP457534LMJ
CSB-BP457534LMJ
CSB-MP457534LMJ
CSB-EP457534LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Putative competence-damage inducible protein (cinA) CSB-YP457535LMJ
CSB-EP457535LMJ
CSB-BP457535LMJ
CSB-MP457535LMJ
CSB-EP457535LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Enolase (eno) CSB-YP457536LMJ
CSB-EP457536LMJ
CSB-BP457536LMJ
CSB-MP457536LMJ
CSB-EP457536LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Probable tRNA sulfurtransferase (thiI) CSB-YP457537LMJ
CSB-EP457537LMJ
CSB-BP457537LMJ
CSB-MP457537LMJ
CSB-EP457537LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei UPF0356 protein LCABL_15300 (LCABL_15300) CSB-YP457538LMJ
CSB-EP457538LMJ
CSB-BP457538LMJ
CSB-MP457538LMJ
CSB-EP457538LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP457539LMJ1
CSB-EP457539LMJ1
CSB-BP457539LMJ1
CSB-MP457539LMJ1
CSB-EP457539LMJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP457540LMJ
CSB-EP457540LMJ
CSB-BP457540LMJ
CSB-MP457540LMJ
CSB-EP457540LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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