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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlorobaculum parvum DNA replication and repair protein recF (recF) CSB-YP457441DSQ
CSB-EP457441DSQ
CSB-BP457441DSQ
CSB-MP457441DSQ
CSB-EP457441DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP457442DSQ
CSB-EP457442DSQ
CSB-BP457442DSQ
CSB-MP457442DSQ
CSB-EP457442DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L14 (rplN) CSB-YP457443DSQ
CSB-EP457443DSQ
CSB-BP457443DSQ
CSB-MP457443DSQ
CSB-EP457443DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 30S ribosomal protein S5 (rpsE) CSB-YP457444DSQ
CSB-EP457444DSQ
CSB-BP457444DSQ
CSB-MP457444DSQ
CSB-EP457444DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 30S ribosomal protein S13 (rpsM) CSB-YP457445DSQ
CSB-EP457445DSQ
CSB-BP457445DSQ
CSB-MP457445DSQ
CSB-EP457445DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Quinolinate synthase A (nadA) CSB-YP457446DSY
CSB-EP457446DSY
CSB-BP457446DSY
CSB-MP457446DSY
CSB-EP457446DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 10 kDa chaperonin (groS) CSB-YP457447DSY
CSB-EP457447DSY
CSB-BP457447DSY
CSB-MP457447DSY
CSB-EP457447DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Shikimate kinase (aroK) CSB-YP457448DSY
CSB-EP457448DSY
CSB-BP457448DSY
CSB-MP457448DSY
CSB-EP457448DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Probable transcriptional regulatory protein Ctha_1786 (Ctha_1786) CSB-YP457449DSY
CSB-EP457449DSY
CSB-BP457449DSY
CSB-MP457449DSY
CSB-EP457449DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium D-tyrosyl-tRNA (Tyr) deacylase CSB-YP457450DSY
CSB-EP457450DSY
CSB-BP457450DSY
CSB-MP457450DSY
CSB-EP457450DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Bifunctional protein FolD (folD) CSB-YP457451DSY
CSB-EP457451DSY
CSB-BP457451DSY
CSB-MP457451DSY
CSB-EP457451DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium NADH-quinone oxidoreductase subunit B 1 (nuoB1) CSB-YP457452DSY
CSB-EP457452DSY
CSB-BP457452DSY
CSB-MP457452DSY
CSB-EP457452DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium NADH-quinone oxidoreductase subunit A 1 (nuoA1), partial CSB-YP457453DSY1
CSB-EP457453DSY1
CSB-BP457453DSY1
CSB-MP457453DSY1
CSB-EP457453DSY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium N utilization substance protein B homolog (nusB) CSB-YP457454DSY
CSB-EP457454DSY
CSB-BP457454DSY
CSB-MP457454DSY
CSB-EP457454DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 30S ribosomal protein S6 (rpsF) CSB-YP457455DSY
CSB-EP457455DSY
CSB-BP457455DSY
CSB-MP457455DSY
CSB-EP457455DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Undecaprenyl-diphosphatase (uppP), partial CSB-YP457456DSY1
CSB-EP457456DSY1
CSB-BP457456DSY1
CSB-MP457456DSY1
CSB-EP457456DSY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Probable pyruvoyl-dependent arginine decarboxylase (pdaD) CSB-YP457457DSY
CSB-EP457457DSY
CSB-BP457457DSY
CSB-MP457457DSY
CSB-EP457457DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP457458DSY
CSB-EP457458DSY
CSB-BP457458DSY
CSB-MP457458DSY
CSB-EP457458DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP457459DSY1
CSB-EP457459DSY1
CSB-BP457459DSY1
CSB-MP457459DSY1
CSB-EP457459DSY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Protein translocase subunit SecA (secA), partial CSB-YP457460DSY
CSB-EP457460DSY
CSB-BP457460DSY
CSB-MP457460DSY
CSB-EP457460DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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