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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus casei D-tyrosyl-tRNA (Tyr) deacylase CSB-YP457541LMJ
CSB-EP457541LMJ
CSB-BP457541LMJ
CSB-MP457541LMJ
CSB-EP457541LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Bifunctional protein FolD (folD) CSB-YP457542LMJ
CSB-EP457542LMJ
CSB-BP457542LMJ
CSB-MP457542LMJ
CSB-EP457542LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Threonine--tRNA ligase (thrS), partial CSB-YP457543LMJ
CSB-EP457543LMJ
CSB-BP457543LMJ
CSB-MP457543LMJ
CSB-EP457543LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP457544LMJ
CSB-EP457544LMJ
CSB-BP457544LMJ
CSB-MP457544LMJ
CSB-EP457544LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein shisa-9 (SHISA9), partial CSB-YP457545HU
CSB-EP457545HU
CSB-BP457545HU
CSB-MP457545HU
CSB-EP457545HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP457546BXS
CSB-EP457546BXS
CSB-BP457546BXS
CSB-MP457546BXS
CSB-EP457546BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 30S ribosomal protein S7 (rpsG) CSB-YP457547BXS
CSB-EP457547BXS
CSB-BP457547BXS
CSB-MP457547BXS
CSB-EP457547BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 50S ribosomal protein L16 (rplP) CSB-YP457548BXS
CSB-EP457548BXS
CSB-BP457548BXS
CSB-MP457548BXS
CSB-EP457548BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 50S ribosomal protein L30 (rpmD) CSB-YP457549BXS
CSB-EP457549BXS
CSB-BP457549BXS
CSB-MP457549BXS
CSB-EP457549BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP457550BXS
CSB-EP457550BXS
CSB-BP457550BXS
CSB-MP457550BXS
CSB-EP457550BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP457551BXS
CSB-EP457551BXS
CSB-BP457551BXS
CSB-MP457551BXS
CSB-EP457551BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Protein translocase subunit SecA (secA), partial CSB-YP457552BXS
CSB-EP457552BXS
CSB-BP457552BXS
CSB-MP457552BXS
CSB-EP457552BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 3-dehydroquinate synthase (aroB) CSB-YP457553BXS
CSB-EP457553BXS
CSB-BP457553BXS
CSB-MP457553BXS
CSB-EP457553BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP457554BXS
CSB-EP457554BXS
CSB-BP457554BXS
CSB-MP457554BXS
CSB-EP457554BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP457555BXS
CSB-EP457555BXS
CSB-BP457555BXS
CSB-MP457555BXS
CSB-EP457555BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Glutamyl-tRNA reductase (hemA) CSB-YP457556BXS
CSB-EP457556BXS
CSB-BP457556BXS
CSB-MP457556BXS
CSB-EP457556BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Membrane protein insertase YidC (yidC), partial CSB-YP457557BXS1
CSB-EP457557BXS1
CSB-BP457557BXS1
CSB-MP457557BXS1
CSB-EP457557BXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia S-adenosylmethionine synthase (metK) CSB-YP457558BXS
CSB-EP457558BXS
CSB-BP457558BXS
CSB-MP457558BXS
CSB-EP457558BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Putative arginyl-tRNA--protein transferase (ate) CSB-YP457559BXS
CSB-EP457559BXS
CSB-BP457559BXS
CSB-MP457559BXS
CSB-EP457559BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia UPF0178 protein BceJ2315_16760 (BceJ2315_16760) CSB-YP457560BXS
CSB-EP457560BXS
CSB-BP457560BXS
CSB-MP457560BXS
CSB-EP457560BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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