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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Polynucleobacter necessarius 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP457841EXM
CSB-EP457841EXM
CSB-BP457841EXM
CSB-MP457841EXM
CSB-EP457841EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Ketol-acid reductoisomerase (ilvC) CSB-YP457842EXM
CSB-EP457842EXM
CSB-BP457842EXM
CSB-MP457842EXM
CSB-EP457842EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP457843EXM1
CSB-EP457843EXM1
CSB-BP457843EXM1
CSB-MP457843EXM1
CSB-EP457843EXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Glutamate--tRNA ligase (gltX) CSB-YP457844EXM
CSB-EP457844EXM
CSB-BP457844EXM
CSB-MP457844EXM
CSB-EP457844EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP457845EXM
CSB-EP457845EXM
CSB-BP457845EXM
CSB-MP457845EXM
CSB-EP457845EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP457846EXM
CSB-EP457846EXM
CSB-BP457846EXM
CSB-MP457846EXM
CSB-EP457846EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Dihydroxy-acid dehydratase (ilvD), partial CSB-YP457847EXM
CSB-EP457847EXM
CSB-BP457847EXM
CSB-MP457847EXM
CSB-EP457847EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Ribosome maturation factor RimM (rimM) CSB-YP457848EXM
CSB-EP457848EXM
CSB-BP457848EXM
CSB-MP457848EXM
CSB-EP457848EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Phosphoglycerate kinase (pgk) CSB-YP457849LOT
CSB-EP457849LOT
CSB-BP457849LOT
CSB-MP457849LOT
CSB-EP457849LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Methionine--tRNA ligase (metG), partial CSB-YP457850LOT
CSB-EP457850LOT
CSB-BP457850LOT
CSB-MP457850LOT
CSB-EP457850LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 3-dehydroquinate dehydratase (aroQ) CSB-YP457851LOT
CSB-EP457851LOT
CSB-BP457851LOT
CSB-MP457851LOT
CSB-EP457851LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L9 (rplI) CSB-YP457852LOT
CSB-EP457852LOT
CSB-BP457852LOT
CSB-MP457852LOT
CSB-EP457852LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 10 kDa chaperonin (groS) CSB-YP457853LOT
CSB-EP457853LOT
CSB-BP457853LOT
CSB-MP457853LOT
CSB-EP457853LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Tryptophan synthase alpha chain (trpA) CSB-YP457854LOT
CSB-EP457854LOT
CSB-BP457854LOT
CSB-MP457854LOT
CSB-EP457854LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP457855LOT
CSB-EP457855LOT
CSB-BP457855LOT
CSB-MP457855LOT
CSB-EP457855LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L35 (rpmI) CSB-YP457856LOT
CSB-EP457856LOT
CSB-BP457856LOT
CSB-MP457856LOT
CSB-EP457856LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L32 (rpmF) CSB-YP457857LOT
CSB-EP457857LOT
CSB-BP457857LOT
CSB-MP457857LOT
CSB-EP457857LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Ribosome maturation factor RimM (rimM) CSB-YP457858LOT
CSB-EP457858LOT
CSB-BP457858LOT
CSB-MP457858LOT
CSB-EP457858LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Protein translocase subunit SecA (secA), partial CSB-YP457859LOT
CSB-EP457859LOT
CSB-BP457859LOT
CSB-MP457859LOT
CSB-EP457859LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii UPF0133 protein Lcho_1975 (Lcho_1975) CSB-YP457860LOT
CSB-EP457860LOT
CSB-BP457860LOT
CSB-MP457860LOT
CSB-EP457860LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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