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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Lipoate-protein ligase A (lplA) CSB-YP458442YAW
CSB-EP458442YAW
CSB-BP458442YAW
CSB-MP458442YAW
CSB-EP458442YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP458443YAW
CSB-EP458443YAW
CSB-BP458443YAW
CSB-MP458443YAW
CSB-EP458443YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L16 (rplP) CSB-YP458444YAW
CSB-EP458444YAW
CSB-BP458444YAW
CSB-MP458444YAW
CSB-EP458444YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L3 (rplC) CSB-YP458445YAW
CSB-EP458445YAW
CSB-BP458445YAW
CSB-MP458445YAW
CSB-EP458445YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glutathione-regulated potassium-efflux system ancillary protein KefG (kefG) CSB-YP458446YAW
CSB-EP458446YAW
CSB-BP458446YAW
CSB-MP458446YAW
CSB-EP458446YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP458447YAW
CSB-EP458447YAW
CSB-BP458447YAW
CSB-MP458447YAW
CSB-EP458447YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Thiosulfate sulfurtransferase glpE (glpE) CSB-YP458448YAW
CSB-EP458448YAW
CSB-BP458448YAW
CSB-MP458448YAW
CSB-EP458448YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB tRNA (guanine-N (1)-)-methyltransferase CSB-YP458449YAW
CSB-EP458449YAW
CSB-BP458449YAW
CSB-MP458449YAW
CSB-EP458449YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Acireductone dioxygenase (mtnD) CSB-YP458450YAW
CSB-EP458450YAW
CSB-BP458450YAW
CSB-MP458450YAW
CSB-EP458450YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP458451YAW
CSB-EP458451YAW
CSB-BP458451YAW
CSB-MP458451YAW
CSB-EP458451YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protease HtpX (htpX), partial CSB-YP458452YAW1
CSB-EP458452YAW1
CSB-BP458452YAW1
CSB-MP458452YAW1
CSB-EP458452YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glutamate 5-kinase (proB) CSB-YP458453YAW
CSB-EP458453YAW
CSB-BP458453YAW
CSB-MP458453YAW
CSB-EP458453YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP458454YAW
CSB-EP458454YAW
CSB-BP458454YAW
CSB-MP458454YAW
CSB-EP458454YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phosphate acyltransferase (plsX) CSB-YP458455YAW
CSB-EP458455YAW
CSB-BP458455YAW
CSB-MP458455YAW
CSB-EP458455YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Cell volume regulation protein A (cvrA), partial CSB-YP458456YAW
CSB-EP458456YAW
CSB-BP458456YAW
CSB-MP458456YAW
CSB-EP458456YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protein FdhE homolog (fdhE) CSB-YP458457YAW
CSB-EP458457YAW
CSB-BP458457YAW
CSB-MP458457YAW
CSB-EP458457YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP458458YAW
CSB-EP458458YAW
CSB-BP458458YAW
CSB-MP458458YAW
CSB-EP458458YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB o-succinylbenzoate synthase (menC) CSB-YP458459YAW
CSB-EP458459YAW
CSB-BP458459YAW
CSB-MP458459YAW
CSB-EP458459YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP458460YAW
CSB-EP458460YAW
CSB-BP458460YAW
CSB-MP458460YAW
CSB-EP458460YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB ATP synthase epsilon chain (atpC) CSB-YP458461YAW
CSB-EP458461YAW
CSB-BP458461YAW
CSB-MP458461YAW
CSB-EP458461YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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