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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Alteromonas macleodii 30S ribosomal protein S8 (rpsH) CSB-YP457781AZX
CSB-EP457781AZX
CSB-BP457781AZX
CSB-MP457781AZX
CSB-EP457781AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 30S ribosomal protein S4 (rpsD) CSB-YP457782AZX
CSB-EP457782AZX
CSB-BP457782AZX
CSB-MP457782AZX
CSB-EP457782AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii FMN-dependent NADH-azoreductase (azoR) CSB-YP457783AZX
CSB-EP457783AZX
CSB-BP457783AZX
CSB-MP457783AZX
CSB-EP457783AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii UPF0133 protein MADE_1013280 (MADE_1013280) CSB-YP457784AZX
CSB-EP457784AZX
CSB-BP457784AZX
CSB-MP457784AZX
CSB-EP457784AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii UPF0391 membrane protein MADE_1011595 (MADE_1011595), partial CSB-YP457785AZX
CSB-EP457785AZX
CSB-BP457785AZX
CSB-MP457785AZX
CSB-EP457785AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Histidine--tRNA ligase (hisS1) CSB-YP457786AZX
CSB-EP457786AZX
CSB-BP457786AZX
CSB-MP457786AZX
CSB-EP457786AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Elongation factor 4 (lepA1), partial CSB-YP457787AZX
CSB-EP457787AZX
CSB-BP457787AZX
CSB-MP457787AZX
CSB-EP457787AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP457788AZX
CSB-EP457788AZX
CSB-BP457788AZX
CSB-MP457788AZX
CSB-EP457788AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Imidazolonepropionase (hutI) CSB-YP457789AZX
CSB-EP457789AZX
CSB-BP457789AZX
CSB-MP457789AZX
CSB-EP457789AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0234 protein yajQ (yajQ) CSB-YP457790ENT
CSB-EP457790ENT
CSB-BP457790ENT
CSB-MP457790ENT
CSB-EP457790ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA 2-selenouridine synthase (selU) CSB-YP457791ENT
CSB-EP457791ENT
CSB-BP457791ENT
CSB-MP457791ENT
CSB-EP457791ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP457792ENT
CSB-EP457792ENT
CSB-BP457792ENT
CSB-MP457792ENT
CSB-EP457792ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP457793ENT
CSB-EP457793ENT
CSB-BP457793ENT
CSB-MP457793ENT
CSB-EP457793ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP457794ENT1
CSB-EP457794ENT1
CSB-BP457794ENT1
CSB-MP457794ENT1
CSB-EP457794ENT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP457795ENT
CSB-EP457795ENT
CSB-BP457795ENT
CSB-MP457795ENT
CSB-EP457795ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Succinylglutamate desuccinylase (astE) CSB-YP457796ENT
CSB-EP457796ENT
CSB-BP457796ENT
CSB-MP457796ENT
CSB-EP457796ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DnaA initiator-associating protein diaA (diaA) CSB-YP457797ENT
CSB-EP457797ENT
CSB-BP457797ENT
CSB-MP457797ENT
CSB-EP457797ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosome maturation factor RimP (rimP) CSB-YP457798ENT
CSB-EP457798ENT
CSB-BP457798ENT
CSB-MP457798ENT
CSB-EP457798ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0059 membrane protein yebN (yebN), partial CSB-YP457799ENT1
CSB-EP457799ENT1
CSB-BP457799ENT1
CSB-MP457799ENT1
CSB-EP457799ENT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP457800ENT
CSB-EP457800ENT
CSB-BP457800ENT
CSB-MP457800ENT
CSB-EP457800ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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