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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. 50S ribosomal protein L27 (rpmA) CSB-YP457822FQA
CSB-EP457822FQA
CSB-BP457822FQA
CSB-MP457822FQA
CSB-EP457822FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glutamate 5-kinase (proB) CSB-YP457823FQA
CSB-EP457823FQA
CSB-BP457823FQA
CSB-MP457823FQA
CSB-EP457823FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UPF0296 protein SYNPCC7002_A1845 (SYNPCC7002_A1845) CSB-YP457824FQA
CSB-EP457824FQA
CSB-BP457824FQA
CSB-MP457824FQA
CSB-EP457824FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L32 (rpmF) CSB-YP457825FQA
CSB-EP457825FQA
CSB-BP457825FQA
CSB-MP457825FQA
CSB-EP457825FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase subunit b 2 (atpF2), partial CSB-YP457826FQA
CSB-EP457826FQA
CSB-BP457826FQA
CSB-MP457826FQA
CSB-EP457826FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP457827EXM
CSB-EP457827EXM
CSB-BP457827EXM
CSB-MP457827EXM
CSB-EP457827EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L13 (rplM) CSB-YP457828EXM
CSB-EP457828EXM
CSB-BP457828EXM
CSB-MP457828EXM
CSB-EP457828EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius ATP synthase gamma chain (atpG) CSB-YP457829EXM
CSB-EP457829EXM
CSB-BP457829EXM
CSB-MP457829EXM
CSB-EP457829EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L34 (rpmH) CSB-YP457830EXM
CSB-EP457830EXM
CSB-BP457830EXM
CSB-MP457830EXM
CSB-EP457830EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 30S ribosomal protein S10 (rpsJ) CSB-YP457831EXM
CSB-EP457831EXM
CSB-BP457831EXM
CSB-MP457831EXM
CSB-EP457831EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L16 (rplP) CSB-YP457832EXM
CSB-EP457832EXM
CSB-BP457832EXM
CSB-MP457832EXM
CSB-EP457832EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L18 (rplR) CSB-YP457833EXM
CSB-EP457833EXM
CSB-BP457833EXM
CSB-MP457833EXM
CSB-EP457833EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Probable GTP-binding protein EngB (engB) CSB-YP457834EXM
CSB-EP457834EXM
CSB-BP457834EXM
CSB-MP457834EXM
CSB-EP457834EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Anthranilate phosphoribosyltransferase (trpD) CSB-YP457835EXM
CSB-EP457835EXM
CSB-BP457835EXM
CSB-MP457835EXM
CSB-EP457835EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP457836EXM
CSB-EP457836EXM
CSB-BP457836EXM
CSB-MP457836EXM
CSB-EP457836EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Ribosomal protein L11 methyltransferase (prmA) CSB-YP457837EXM
CSB-EP457837EXM
CSB-BP457837EXM
CSB-MP457837EXM
CSB-EP457837EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP457838EXM
CSB-EP457838EXM
CSB-BP457838EXM
CSB-MP457838EXM
CSB-EP457838EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L9 (rplI) CSB-YP457839EXM
CSB-EP457839EXM
CSB-BP457839EXM
CSB-MP457839EXM
CSB-EP457839EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP457840EXM
CSB-EP457840EXM
CSB-BP457840EXM
CSB-MP457840EXM
CSB-EP457840EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP457841EXM
CSB-EP457841EXM
CSB-BP457841EXM
CSB-MP457841EXM
CSB-EP457841EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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