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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella abortus 50S ribosomal protein L14 (rplN) CSB-YP458561BWR
CSB-EP458561BWR
CSB-BP458561BWR
CSB-MP458561BWR
CSB-EP458561BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L2 (rplB) CSB-YP458562BWR
CSB-EP458562BWR
CSB-BP458562BWR
CSB-MP458562BWR
CSB-EP458562BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP458563BWR
CSB-EP458563BWR
CSB-BP458563BWR
CSB-MP458563BWR
CSB-EP458563BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ketol-acid reductoisomerase (ilvC) CSB-YP458564BWR
CSB-EP458564BWR
CSB-BP458564BWR
CSB-MP458564BWR
CSB-EP458564BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP458565BWR
CSB-EP458565BWR
CSB-BP458565BWR
CSB-MP458565BWR
CSB-EP458565BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Cell cycle response regulator CtrA (ctrA) CSB-YP458566BWR
CSB-EP458566BWR
CSB-BP458566BWR
CSB-MP458566BWR
CSB-EP458566BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Protein TolB (tolB) CSB-YP458567BWR
CSB-EP458567BWR
CSB-BP458567BWR
CSB-MP458567BWR
CSB-EP458567BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP458568BWR
CSB-EP458568BWR
CSB-BP458568BWR
CSB-MP458568BWR
CSB-EP458568BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Leucine--tRNA ligase (leuS), partial CSB-YP458569BWR
CSB-EP458569BWR
CSB-BP458569BWR
CSB-MP458569BWR
CSB-EP458569BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus UPF0133 protein BAbS19_I00290 (BAbS19_I00290) CSB-YP458570BWR
CSB-EP458570BWR
CSB-BP458570BWR
CSB-MP458570BWR
CSB-EP458570BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L27 (rpmA) CSB-YP458571BWR
CSB-EP458571BWR
CSB-BP458571BWR
CSB-MP458571BWR
CSB-EP458571BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ribosome maturation factor RimM (rimM) CSB-YP458572BWR
CSB-EP458572BWR
CSB-BP458572BWR
CSB-MP458572BWR
CSB-EP458572BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L28 (rpmB) CSB-YP458573BWR
CSB-EP458573BWR
CSB-BP458573BWR
CSB-MP458573BWR
CSB-EP458573BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP458574BWR
CSB-EP458574BWR
CSB-BP458574BWR
CSB-MP458574BWR
CSB-EP458574BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP458575BWR
CSB-EP458575BWR
CSB-BP458575BWR
CSB-MP458575BWR
CSB-EP458575BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ribosome-binding factor A (rbfA) CSB-YP458576BWR
CSB-EP458576BWR
CSB-BP458576BWR
CSB-MP458576BWR
CSB-EP458576BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Pantothenate synthetase (panC) CSB-YP458577BWR
CSB-EP458577BWR
CSB-BP458577BWR
CSB-MP458577BWR
CSB-EP458577BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP458578BWR
CSB-EP458578BWR
CSB-BP458578BWR
CSB-MP458578BWR
CSB-EP458578BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L9 (rplI) CSB-YP458579BWR
CSB-EP458579BWR
CSB-BP458579BWR
CSB-MP458579BWR
CSB-EP458579BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP458580BWR
CSB-EP458580BWR
CSB-BP458580BWR
CSB-MP458580BWR
CSB-EP458580BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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