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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP458421YAW
CSB-EP458421YAW
CSB-BP458421YAW
CSB-MP458421YAW
CSB-EP458421YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phosphoglucosamine mutase (glmM) CSB-YP458422YAW
CSB-EP458422YAW
CSB-BP458422YAW
CSB-MP458422YAW
CSB-EP458422YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP458423YAW
CSB-EP458423YAW
CSB-BP458423YAW
CSB-MP458423YAW
CSB-EP458423YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Aspartate--tRNA ligase (aspS), partial CSB-YP458424YAW
CSB-EP458424YAW
CSB-BP458424YAW
CSB-MP458424YAW
CSB-EP458424YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Autoinducer 2-binding protein lsrB (lsrB) CSB-YP458425YAW
CSB-EP458425YAW
CSB-BP458425YAW
CSB-MP458425YAW
CSB-EP458425YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0391 membrane protein YPTS_0599 (YPTS_0599) CSB-YP458426YAW
CSB-EP458426YAW
CSB-BP458426YAW
CSB-MP458426YAW
CSB-EP458426YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Chaperone protein DnaJ (dnaJ) CSB-YP458427YAW
CSB-EP458427YAW
CSB-BP458427YAW
CSB-MP458427YAW
CSB-EP458427YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Probable septum site-determining protein MinC (minC) CSB-YP458428YAW
CSB-EP458428YAW
CSB-BP458428YAW
CSB-MP458428YAW
CSB-EP458428YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0229 protein YPTS_2141 (YPTS_2141) CSB-YP458429YAW
CSB-EP458429YAW
CSB-BP458429YAW
CSB-MP458429YAW
CSB-EP458429YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0259 membrane protein YPTS_2193 (YPTS_2193) CSB-YP458430YAW
CSB-EP458430YAW
CSB-BP458430YAW
CSB-MP458430YAW
CSB-EP458430YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB DnaA initiator-associating protein diaA (diaA) CSB-YP458431YAW
CSB-EP458431YAW
CSB-BP458431YAW
CSB-MP458431YAW
CSB-EP458431YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP458432YAW
CSB-EP458432YAW
CSB-BP458432YAW
CSB-MP458432YAW
CSB-EP458432YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 3-dehydroquinate dehydratase (aroQ) CSB-YP458433YAW
CSB-EP458433YAW
CSB-BP458433YAW
CSB-MP458433YAW
CSB-EP458433YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP458434YAW
CSB-EP458434YAW
CSB-BP458434YAW
CSB-MP458434YAW
CSB-EP458434YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protein translocase subunit SecA (secA), partial CSB-YP458435YAW
CSB-EP458435YAW
CSB-BP458435YAW
CSB-MP458435YAW
CSB-EP458435YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Electron transport complex protein RnfE (rnfE), partial CSB-YP458436YAW1
CSB-EP458436YAW1
CSB-BP458436YAW1
CSB-MP458436YAW1
CSB-EP458436YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Alkanesulfonate monooxygenase (ssuD) CSB-YP458437YAW
CSB-EP458437YAW
CSB-BP458437YAW
CSB-MP458437YAW
CSB-EP458437YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 30S ribosomal protein S13 (rpsM) CSB-YP458438YAW
CSB-EP458438YAW
CSB-BP458438YAW
CSB-MP458438YAW
CSB-EP458438YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Aspartate 1-decarboxylase (panD) CSB-YP458439YAW
CSB-EP458439YAW
CSB-BP458439YAW
CSB-MP458439YAW
CSB-EP458439YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP458440YAW
CSB-EP458440YAW
CSB-BP458440YAW
CSB-MP458440YAW
CSB-EP458440YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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