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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia phymatum D-alanine--D-alanine ligase (ddl) CSB-YP458381BXT
CSB-EP458381BXT
CSB-BP458381BXT
CSB-MP458381BXT
CSB-EP458381BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Probable GTP-binding protein EngB (engB) CSB-YP458382BXT
CSB-EP458382BXT
CSB-BP458382BXT
CSB-MP458382BXT
CSB-EP458382BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 30S ribosomal protein S5 (rpsE) CSB-YP458383BXT
CSB-EP458383BXT
CSB-BP458383BXT
CSB-MP458383BXT
CSB-EP458383BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 30S ribosomal protein S19 (rpsS) CSB-YP458384BXT
CSB-EP458384BXT
CSB-BP458384BXT
CSB-MP458384BXT
CSB-EP458384BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP458385BXT
CSB-EP458385BXT
CSB-BP458385BXT
CSB-MP458385BXT
CSB-EP458385BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP458386BXT
CSB-EP458386BXT
CSB-BP458386BXT
CSB-MP458386BXT
CSB-EP458386BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L3 (rplC) CSB-YP458387BXT
CSB-EP458387BXT
CSB-BP458387BXT
CSB-MP458387BXT
CSB-EP458387BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L7/L12 (rplL) CSB-YP458388BXT
CSB-EP458388BXT
CSB-BP458388BXT
CSB-MP458388BXT
CSB-EP458388BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Multifunctional CCA protein (cca) CSB-YP458389BXT
CSB-EP458389BXT
CSB-BP458389BXT
CSB-MP458389BXT
CSB-EP458389BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP458390BXT
CSB-EP458390BXT
CSB-BP458390BXT
CSB-MP458390BXT
CSB-EP458390BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Lysine--tRNA ligase (lysS), partial CSB-YP458391BXT
CSB-EP458391BXT
CSB-BP458391BXT
CSB-MP458391BXT
CSB-EP458391BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Probable Fe (2+)-trafficking protein CSB-YP458392BXT
CSB-EP458392BXT
CSB-BP458392BXT
CSB-MP458392BXT
CSB-EP458392BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Homoserine O-acetyltransferase (metX) CSB-YP458393BXT
CSB-EP458393BXT
CSB-BP458393BXT
CSB-MP458393BXT
CSB-EP458393BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum LexA repressor (lexA) CSB-YP458394BXT
CSB-EP458394BXT
CSB-BP458394BXT
CSB-MP458394BXT
CSB-EP458394BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP458395BXT
CSB-EP458395BXT
CSB-BP458395BXT
CSB-MP458395BXT
CSB-EP458395BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum L-rhamnose mutarotase (rhaM) CSB-YP458396BXT
CSB-EP458396BXT
CSB-BP458396BXT
CSB-MP458396BXT
CSB-EP458396BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UPF0060 membrane protein Bphy_5052 (Bphy_5052), partial CSB-YP458397BXT1
CSB-EP458397BXT1
CSB-BP458397BXT1
CSB-MP458397BXT1
CSB-EP458397BXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP458398BXT
CSB-EP458398BXT
CSB-BP458398BXT
CSB-MP458398BXT
CSB-EP458398BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Tryptophan synthase alpha chain (trpA) CSB-YP458399BXT
CSB-EP458399BXT
CSB-BP458399BXT
CSB-MP458399BXT
CSB-EP458399BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP458400BXT
CSB-EP458400BXT
CSB-BP458400BXT
CSB-MP458400BXT
CSB-EP458400BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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