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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Porphyromonas gingivalis Copper homeostasis protein CutC (cutC) CSB-YP458502EXZ
CSB-EP458502EXZ
CSB-BP458502EXZ
CSB-MP458502EXZ
CSB-EP458502EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Leucine--tRNA ligase (leuS), partial CSB-YP458503EXZ
CSB-EP458503EXZ
CSB-BP458503EXZ
CSB-MP458503EXZ
CSB-EP458503EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP458504EXZ
CSB-EP458504EXZ
CSB-BP458504EXZ
CSB-MP458504EXZ
CSB-EP458504EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP458505EXZ
CSB-EP458505EXZ
CSB-BP458505EXZ
CSB-MP458505EXZ
CSB-EP458505EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Acetate kinase (ackA) CSB-YP458506EXZ
CSB-EP458506EXZ
CSB-BP458506EXZ
CSB-MP458506EXZ
CSB-EP458506EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Elongation factor 4 (lepA), partial CSB-YP458507EXZ
CSB-EP458507EXZ
CSB-BP458507EXZ
CSB-MP458507EXZ
CSB-EP458507EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis DNA mismatch repair protein mutL (mutL), partial CSB-YP458508EXZ
CSB-EP458508EXZ
CSB-BP458508EXZ
CSB-MP458508EXZ
CSB-EP458508EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L11 (rplK) CSB-YP458509EXZ
CSB-EP458509EXZ
CSB-BP458509EXZ
CSB-MP458509EXZ
CSB-EP458509EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 30S ribosomal protein S9 (rpsI) CSB-YP458510EXZ
CSB-EP458510EXZ
CSB-BP458510EXZ
CSB-MP458510EXZ
CSB-EP458510EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis S-adenosylmethionine synthase (metK) CSB-YP458511EXZ
CSB-EP458511EXZ
CSB-BP458511EXZ
CSB-MP458511EXZ
CSB-EP458511EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L6 (rplF) CSB-YP458512EXZ
CSB-EP458512EXZ
CSB-BP458512EXZ
CSB-MP458512EXZ
CSB-EP458512EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L14 (rplN) CSB-YP458513EXZ
CSB-EP458513EXZ
CSB-BP458513EXZ
CSB-MP458513EXZ
CSB-EP458513EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 30S ribosomal protein S10 (rpsJ) CSB-YP458514EXZ
CSB-EP458514EXZ
CSB-BP458514EXZ
CSB-MP458514EXZ
CSB-EP458514EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis DNA protection during starvation protein (dps) CSB-YP458515EXZ
CSB-EP458515EXZ
CSB-BP458515EXZ
CSB-MP458515EXZ
CSB-EP458515EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rho GTPase-activating protein 42 (Arhgap42), partial CSB-YP458516MO
CSB-EP458516MO
CSB-BP458516MO
CSB-MP458516MO
CSB-EP458516MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human von Willebrand factor C domain-containing protein 2-like (VWC2L) CSB-YP458517HU
CSB-EP458517HU
CSB-BP458517HU
CSB-MP458517HU
CSB-EP458517HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protein 229A (TMEM229A), partial CSB-YP458518HU1
CSB-EP458518HU1
CSB-BP458518HU1
CSB-MP458518HU1
CSB-EP458518HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Glucosamine-6-phosphate deaminase (nagB) CSB-YP458519BUJ
CSB-EP458519BUJ
CSB-BP458519BUJ
CSB-MP458519BUJ
CSB-EP458519BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Undecaprenyl-diphosphatase (uppP), partial CSB-YP458520BUJ
CSB-EP458520BUJ
CSB-BP458520BUJ
CSB-MP458520BUJ
CSB-EP458520BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 30S ribosomal protein S9 (rpsI) CSB-YP458521BUJ
CSB-EP458521BUJ
CSB-BP458521BUJ
CSB-MP458521BUJ
CSB-EP458521BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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