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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 3-dehydroquinate synthase (aroB) CSB-YP459202AXI
CSB-EP459202AXI
CSB-BP459202AXI
CSB-MP459202AXI
CSB-EP459202AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP459203AXI
CSB-EP459203AXI
CSB-BP459203AXI
CSB-MP459203AXI
CSB-EP459203AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Autonomous glycyl radical cofactor (grcA) CSB-YP459204AXI
CSB-EP459204AXI
CSB-BP459204AXI
CSB-MP459204AXI
CSB-EP459204AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP459205AXI
CSB-EP459205AXI
CSB-BP459205AXI
CSB-MP459205AXI
CSB-EP459205AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Chromosome partition protein MukB (mukB), partial CSB-YP459206AXI
CSB-EP459206AXI
CSB-BP459206AXI
CSB-MP459206AXI
CSB-EP459206AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosome maturation factor RimP (rimP) CSB-YP459207AXI
CSB-EP459207AXI
CSB-BP459207AXI
CSB-MP459207AXI
CSB-EP459207AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Alanine--tRNA ligase (alaS), partial CSB-YP459208AXI
CSB-EP459208AXI
CSB-BP459208AXI
CSB-MP459208AXI
CSB-EP459208AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP459209AXI
CSB-EP459209AXI
CSB-BP459209AXI
CSB-MP459209AXI
CSB-EP459209AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Peptidase E (pepE) CSB-YP459210AXI
CSB-EP459210AXI
CSB-BP459210AXI
CSB-MP459210AXI
CSB-EP459210AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S15 (rpsO) CSB-YP459211AXI
CSB-EP459211AXI
CSB-BP459211AXI
CSB-MP459211AXI
CSB-EP459211AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UPF0234 protein APP7_1280 (APP7_1280) CSB-YP459212AXI
CSB-EP459212AXI
CSB-BP459212AXI
CSB-MP459212AXI
CSB-EP459212AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Adenylate kinase (adk) CSB-YP459213AXI
CSB-EP459213AXI
CSB-BP459213AXI
CSB-MP459213AXI
CSB-EP459213AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP459214AXI
CSB-EP459214AXI
CSB-BP459214AXI
CSB-MP459214AXI
CSB-EP459214AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S12 (rpsL) CSB-YP459215AXI
CSB-EP459215AXI
CSB-BP459215AXI
CSB-MP459215AXI
CSB-EP459215AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP459216AXI
CSB-EP459216AXI
CSB-BP459216AXI
CSB-MP459216AXI
CSB-EP459216AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Octanoyltransferase (lipB) CSB-YP459217AXI
CSB-EP459217AXI
CSB-BP459217AXI
CSB-MP459217AXI
CSB-EP459217AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP459218AXI
CSB-EP459218AXI
CSB-BP459218AXI
CSB-MP459218AXI
CSB-EP459218AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP459219AXI
CSB-EP459219AXI
CSB-BP459219AXI
CSB-MP459219AXI
CSB-EP459219AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Regulator of ribonuclease activity A (rraA) CSB-YP459220AXI
CSB-EP459220AXI
CSB-BP459220AXI
CSB-MP459220AXI
CSB-EP459220AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At4g35733 (At4g35733) CSB-YP459221DOA
CSB-EP459221DOA
CSB-BP459221DOA
CSB-MP459221DOA
CSB-EP459221DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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