Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP459181AXI
CSB-EP459181AXI
CSB-BP459181AXI
CSB-MP459181AXI
CSB-EP459181AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Beta-hexosaminidase (nagZ) CSB-YP459182AXI
CSB-EP459182AXI
CSB-BP459182AXI
CSB-MP459182AXI
CSB-EP459182AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP459183AXI
CSB-EP459183AXI
CSB-BP459183AXI
CSB-MP459183AXI
CSB-EP459183AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 33 kDa chaperonin (hslO) CSB-YP459184AXI
CSB-EP459184AXI
CSB-BP459184AXI
CSB-MP459184AXI
CSB-EP459184AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L32 (rpmF) CSB-YP459185AXI
CSB-EP459185AXI
CSB-BP459185AXI
CSB-MP459185AXI
CSB-EP459185AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Fumarate reductase subunit D (frdD), partial CSB-YP459186AXI1
CSB-EP459186AXI1
CSB-BP459186AXI1
CSB-MP459186AXI1
CSB-EP459186AXI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP459187AXI
CSB-EP459187AXI
CSB-BP459187AXI
CSB-MP459187AXI
CSB-EP459187AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L11 (rplK) CSB-YP459188AXI
CSB-EP459188AXI
CSB-BP459188AXI
CSB-MP459188AXI
CSB-EP459188AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L2 (rplB) CSB-YP459189AXI
CSB-EP459189AXI
CSB-BP459189AXI
CSB-MP459189AXI
CSB-EP459189AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S17 (rpsQ) CSB-YP459190AXI
CSB-EP459190AXI
CSB-BP459190AXI
CSB-MP459190AXI
CSB-EP459190AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S8 (rpsH) CSB-YP459191AXI
CSB-EP459191AXI
CSB-BP459191AXI
CSB-MP459191AXI
CSB-EP459191AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S16 (rpsP) CSB-YP459192AXI
CSB-EP459192AXI
CSB-BP459192AXI
CSB-MP459192AXI
CSB-EP459192AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP459193AXI
CSB-EP459193AXI
CSB-BP459193AXI
CSB-MP459193AXI
CSB-EP459193AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP459194AXI
CSB-EP459194AXI
CSB-BP459194AXI
CSB-MP459194AXI
CSB-EP459194AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 tRNA (uracil (54)-C (5))-methyltransferase CSB-YP459195AXI
CSB-EP459195AXI
CSB-BP459195AXI
CSB-MP459195AXI
CSB-EP459195AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP459196AXI
CSB-EP459196AXI
CSB-BP459196AXI
CSB-MP459196AXI
CSB-EP459196AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP459197AXI
CSB-EP459197AXI
CSB-BP459197AXI
CSB-MP459197AXI
CSB-EP459197AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Protein MraZ (mraZ) CSB-YP459198AXI
CSB-EP459198AXI
CSB-BP459198AXI
CSB-MP459198AXI
CSB-EP459198AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 D-alanine--D-alanine ligase (ddl) CSB-YP459199AXI
CSB-EP459199AXI
CSB-BP459199AXI
CSB-MP459199AXI
CSB-EP459199AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribonuclease PH (rph) CSB-YP459200AXI
CSB-EP459200AXI
CSB-BP459200AXI
CSB-MP459200AXI
CSB-EP459200AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>