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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila erecta tRNA (guanine-N (7)-)-methyltransferase subunit wuho CSB-YP459261DLK
CSB-EP459261DLK
CSB-BP459261DLK
CSB-MP459261DLK
CSB-EP459261DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Eukaryotic translation initiation factor 3 subunit G-2 (eIF3-S4-2) CSB-YP459262DLK
CSB-EP459262DLK
CSB-BP459262DLK
CSB-MP459262DLK
CSB-EP459262DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Eukaryotic translation initiation factor 3 subunit F-1 (eIF3-S5-1) CSB-YP459263DLK
CSB-EP459263DLK
CSB-BP459263DLK
CSB-MP459263DLK
CSB-EP459263DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein Turandot B2 (TotB2) CSB-YP459264DLK
CSB-EP459264DLK
CSB-BP459264DLK
CSB-MP459264DLK
CSB-EP459264DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Probable ATP-dependent RNA helicase spindle-E (spn-E), partial CSB-YP459265DLK
CSB-EP459265DLK
CSB-BP459265DLK
CSB-MP459265DLK
CSB-EP459265DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Molybdopterin synthase sulfur carrier subunit (Mocs2) CSB-YP459266DLK
CSB-EP459266DLK
CSB-BP459266DLK
CSB-MP459266DLK
CSB-EP459266DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Eukaryotic translation initiation factor 3 subunit D-1 (eIF-3p66), partial CSB-YP459267DLK
CSB-EP459267DLK
CSB-BP459267DLK
CSB-MP459267DLK
CSB-EP459267DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Electron transport complex protein RnfD (rnfD), partial CSB-YP459268DQY
CSB-EP459268DQY
CSB-BP459268DQY
CSB-MP459268DQY
CSB-EP459268DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Uronate isomerase (uxaC) CSB-YP459269DQY
CSB-EP459269DQY
CSB-BP459269DQY
CSB-MP459269DQY
CSB-EP459269DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP459270DQY
CSB-EP459270DQY
CSB-BP459270DQY
CSB-MP459270DQY
CSB-EP459270DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Catalase-peroxidase 1 (katG1), partial CSB-YP459271DQY
CSB-EP459271DQY
CSB-BP459271DQY
CSB-MP459271DQY
CSB-EP459271DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP459272DQY
CSB-EP459272DQY
CSB-BP459272DQY
CSB-MP459272DQY
CSB-EP459272DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus GTPase Der (der) CSB-YP459273DQY
CSB-EP459273DQY
CSB-BP459273DQY
CSB-MP459273DQY
CSB-EP459273DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP459274DQY
CSB-EP459274DQY
CSB-BP459274DQY
CSB-MP459274DQY
CSB-EP459274DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Diaminopimelate epimerase (dapF) CSB-YP459275DQY
CSB-EP459275DQY
CSB-BP459275DQY
CSB-MP459275DQY
CSB-EP459275DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP459276DQY
CSB-EP459276DQY
CSB-BP459276DQY
CSB-MP459276DQY
CSB-EP459276DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus 50S ribosomal protein L28 (rpmB) CSB-YP459277DQY
CSB-EP459277DQY
CSB-BP459277DQY
CSB-MP459277DQY
CSB-EP459277DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Orotate phosphoribosyltransferase (pyrE) CSB-YP459278DQY
CSB-EP459278DQY
CSB-BP459278DQY
CSB-MP459278DQY
CSB-EP459278DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP459279DQY
CSB-EP459279DQY
CSB-BP459279DQY
CSB-MP459279DQY
CSB-EP459279DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP459280DQY
CSB-EP459280DQY
CSB-BP459280DQY
CSB-MP459280DQY
CSB-EP459280DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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