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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechococcus sp. Arginine--tRNA ligase (argS), partial CSB-YP459781FQA
CSB-EP459781FQA
CSB-BP459781FQA
CSB-MP459781FQA
CSB-EP459781FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP459782FQA
CSB-EP459782FQA
CSB-BP459782FQA
CSB-MP459782FQA
CSB-EP459782FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 10 kDa chaperonin (groS) CSB-YP459783EXM
CSB-EP459783EXM
CSB-BP459783EXM
CSB-MP459783EXM
CSB-EP459783EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius UPF0042 nucleotide-binding protein Pnec_1620 (Pnec_1620) CSB-YP459784EXM
CSB-EP459784EXM
CSB-BP459784EXM
CSB-MP459784EXM
CSB-EP459784EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP459785EXM
CSB-EP459785EXM
CSB-BP459785EXM
CSB-MP459785EXM
CSB-EP459785EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius ATP synthase epsilon chain (atpC) CSB-YP459786EXM
CSB-EP459786EXM
CSB-BP459786EXM
CSB-MP459786EXM
CSB-EP459786EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Orotate phosphoribosyltransferase (pyrE) CSB-YP459787EXM
CSB-EP459787EXM
CSB-BP459787EXM
CSB-MP459787EXM
CSB-EP459787EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L7/L12 (rplL) CSB-YP459788EXM
CSB-EP459788EXM
CSB-BP459788EXM
CSB-MP459788EXM
CSB-EP459788EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L4 (rplD) CSB-YP459789EXM
CSB-EP459789EXM
CSB-BP459789EXM
CSB-MP459789EXM
CSB-EP459789EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L14 (rplN) CSB-YP459790EXM
CSB-EP459790EXM
CSB-BP459790EXM
CSB-MP459790EXM
CSB-EP459790EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L15 (rplO) CSB-YP459791EXM
CSB-EP459791EXM
CSB-BP459791EXM
CSB-MP459791EXM
CSB-EP459791EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP459792EXM
CSB-EP459792EXM
CSB-BP459792EXM
CSB-MP459792EXM
CSB-EP459792EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP459793EXM
CSB-EP459793EXM
CSB-BP459793EXM
CSB-MP459793EXM
CSB-EP459793EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L27 (rpmA) CSB-YP459794EXM
CSB-EP459794EXM
CSB-BP459794EXM
CSB-MP459794EXM
CSB-EP459794EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP459795EXM
CSB-EP459795EXM
CSB-BP459795EXM
CSB-MP459795EXM
CSB-EP459795EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP459796EXM
CSB-EP459796EXM
CSB-BP459796EXM
CSB-MP459796EXM
CSB-EP459796EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius DNA repair protein recO (recO) CSB-YP459797EXM
CSB-EP459797EXM
CSB-BP459797EXM
CSB-MP459797EXM
CSB-EP459797EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Lysine--tRNA ligase (lysS), partial CSB-YP459798EXM
CSB-EP459798EXM
CSB-BP459798EXM
CSB-MP459798EXM
CSB-EP459798EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP459799EXM
CSB-EP459799EXM
CSB-BP459799EXM
CSB-MP459799EXM
CSB-EP459799EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Ribose-5-phosphate isomerase A (rpiA) CSB-YP459800EXM
CSB-EP459800EXM
CSB-BP459800EXM
CSB-MP459800EXM
CSB-EP459800EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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