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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Natranaerobius thermophilus 50S ribosomal protein L2 (rplB) CSB-YP459982NFO
CSB-EP459982NFO
CSB-BP459982NFO
CSB-MP459982NFO
CSB-EP459982NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 50S ribosomal protein L5 (rplE) CSB-YP459983NFO
CSB-EP459983NFO
CSB-BP459983NFO
CSB-MP459983NFO
CSB-EP459983NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Deoxyribose-phosphate aldolase (deoC) CSB-YP459984NFO
CSB-EP459984NFO
CSB-BP459984NFO
CSB-MP459984NFO
CSB-EP459984NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 30S ribosomal protein S4 (rpsD) CSB-YP459985NFO
CSB-EP459985NFO
CSB-BP459985NFO
CSB-MP459985NFO
CSB-EP459985NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP459986NFO
CSB-EP459986NFO
CSB-BP459986NFO
CSB-MP459986NFO
CSB-EP459986NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP459987NFO
CSB-EP459987NFO
CSB-BP459987NFO
CSB-MP459987NFO
CSB-EP459987NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP459988NFO
CSB-EP459988NFO
CSB-BP459988NFO
CSB-MP459988NFO
CSB-EP459988NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Proline--tRNA ligase (proS) CSB-YP459989NFO
CSB-EP459989NFO
CSB-BP459989NFO
CSB-MP459989NFO
CSB-EP459989NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus UPF0178 protein Nther_1836 (Nther_1836) CSB-YP459990NFO
CSB-EP459990NFO
CSB-BP459990NFO
CSB-MP459990NFO
CSB-EP459990NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Leucine--tRNA ligase (leuS), partial CSB-YP459991NFO
CSB-EP459991NFO
CSB-BP459991NFO
CSB-MP459991NFO
CSB-EP459991NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Flagellar assembly factor FliW (fliW) CSB-YP459992NFO
CSB-EP459992NFO
CSB-BP459992NFO
CSB-MP459992NFO
CSB-EP459992NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus UPF0173 metal-dependent hydrolase Nther_2337 (Nther_2337) CSB-YP459993NFO
CSB-EP459993NFO
CSB-BP459993NFO
CSB-MP459993NFO
CSB-EP459993NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis UPF0271 protein RALTA_A0068 (RALTA_A0068) CSB-YP459994DZS
CSB-EP459994DZS
CSB-BP459994DZS
CSB-MP459994DZS
CSB-EP459994DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis ATP-dependent protease subunit HslV (hslV) CSB-YP459995DZS
CSB-EP459995DZS
CSB-BP459995DZS
CSB-MP459995DZS
CSB-EP459995DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Protein-export protein secB (secB) CSB-YP459996DZS
CSB-EP459996DZS
CSB-BP459996DZS
CSB-MP459996DZS
CSB-EP459996DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP459997DZS
CSB-EP459997DZS
CSB-BP459997DZS
CSB-MP459997DZS
CSB-EP459997DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP459998DZS
CSB-EP459998DZS
CSB-BP459998DZS
CSB-MP459998DZS
CSB-EP459998DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Flagellar L-ring protein (flgH) CSB-YP459999DZS
CSB-EP459999DZS
CSB-BP459999DZS
CSB-MP459999DZS
CSB-EP459999DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Glutamyl-tRNA (Gln) amidotransferase subunit B, mitochondrial, partial CSB-YP460000EXJ
CSB-EP460000EXJ
CSB-BP460000EXJ
CSB-MP460000EXJ
CSB-EP460000EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina tRNA (guanine-N (7)-)-methyltransferase CSB-YP460001EXJ
CSB-EP460001EXJ
CSB-BP460001EXJ
CSB-MP460001EXJ
CSB-EP460001EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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