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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP460381YAW
CSB-EP460381YAW
CSB-BP460381YAW
CSB-MP460381YAW
CSB-EP460381YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP460382YAW
CSB-EP460382YAW
CSB-BP460382YAW
CSB-MP460382YAW
CSB-EP460382YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0482 protein YPTS_2265/YPTS_2268 (YPTS_2265) CSB-YP460383YAW
CSB-EP460383YAW
CSB-BP460383YAW
CSB-MP460383YAW
CSB-EP460383YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L30 (rpmD) CSB-YP460384YAW
CSB-EP460384YAW
CSB-BP460384YAW
CSB-MP460384YAW
CSB-EP460384YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP460385YAW
CSB-EP460385YAW
CSB-BP460385YAW
CSB-MP460385YAW
CSB-EP460385YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 5'/3'-nucleotidase SurE (surE) CSB-YP460386YAW
CSB-EP460386YAW
CSB-BP460386YAW
CSB-MP460386YAW
CSB-EP460386YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Catalase-peroxidase (katG), partial CSB-YP460387YAW
CSB-EP460387YAW
CSB-BP460387YAW
CSB-MP460387YAW
CSB-EP460387YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Putative phosphotransferase YPTS_2394 (YPTS_2394) CSB-YP460388YAW
CSB-EP460388YAW
CSB-BP460388YAW
CSB-MP460388YAW
CSB-EP460388YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP460389YAW
CSB-EP460389YAW
CSB-BP460389YAW
CSB-MP460389YAW
CSB-EP460389YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L14 (rplN) CSB-YP460390YAW
CSB-EP460390YAW
CSB-BP460390YAW
CSB-MP460390YAW
CSB-EP460390YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 30S ribosomal protein S19 (rpsS) CSB-YP460391YAW
CSB-EP460391YAW
CSB-BP460391YAW
CSB-MP460391YAW
CSB-EP460391YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0270 protein YPTS_3917 (YPTS_3917) CSB-YP460392YAW
CSB-EP460392YAW
CSB-BP460392YAW
CSB-MP460392YAW
CSB-EP460392YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB HTH-type transcriptional regulator malT (malT), partial CSB-YP460393YAW
CSB-EP460393YAW
CSB-BP460393YAW
CSB-MP460393YAW
CSB-EP460393YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Ribosome maturation factor RimM (rimM) CSB-YP460394YAW
CSB-EP460394YAW
CSB-BP460394YAW
CSB-MP460394YAW
CSB-EP460394YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L35 (rpmI) CSB-YP460395YAW
CSB-EP460395YAW
CSB-BP460395YAW
CSB-MP460395YAW
CSB-EP460395YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glycogen synthase (glgA) CSB-YP460396YAW
CSB-EP460396YAW
CSB-BP460396YAW
CSB-MP460396YAW
CSB-EP460396YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB C4-dicarboxylate transport protein (dctA), partial CSB-YP460397YAW
CSB-EP460397YAW
CSB-BP460397YAW
CSB-MP460397YAW
CSB-EP460397YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB N utilization substance protein B homolog (nusB) CSB-YP460398YAW
CSB-EP460398YAW
CSB-BP460398YAW
CSB-MP460398YAW
CSB-EP460398YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 7-cyano-7-deazaguanine synthase (queC) CSB-YP460399YAW
CSB-EP460399YAW
CSB-BP460399YAW
CSB-MP460399YAW
CSB-EP460399YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0133 protein YPTS_1036 (YPTS_1036) CSB-YP460400YAW
CSB-EP460400YAW
CSB-BP460400YAW
CSB-MP460400YAW
CSB-EP460400YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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