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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP461181SVP
CSB-EP461181SVP
CSB-BP461181SVP
CSB-MP461181SVP
CSB-EP461181SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae SWI5-dependent HO expression protein 2 (SHE2) CSB-YP461182SVP
CSB-EP461182SVP
CSB-BP461182SVP
CSB-MP461182SVP
CSB-EP461182SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Topoisomerase I damage affected protein 2 (TDA2) CSB-YP461183SVP
CSB-EP461183SVP
CSB-BP461183SVP
CSB-MP461183SVP
CSB-EP461183SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Oxidant-induced cell-cycle arrest protein 5 (OCA5), partial CSB-YP461184SVP
CSB-EP461184SVP
CSB-BP461184SVP
CSB-MP461184SVP
CSB-EP461184SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribosomal N-lysine methyltransferase 5 (RKM5) CSB-YP461185SVP
CSB-EP461185SVP
CSB-BP461185SVP
CSB-MP461185SVP
CSB-EP461185SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 21 (AIM21), partial CSB-YP461186SVP
CSB-EP461186SVP
CSB-BP461186SVP
CSB-MP461186SVP
CSB-EP461186SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pheromone-regulated membrane protein 5 (PRM5), partial CSB-YP461187SVP1
CSB-EP461187SVP1
CSB-BP461187SVP1
CSB-MP461187SVP1
CSB-EP461187SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Transmembrane emp24 domain-containing protein bai (bai), partial CSB-YP461188DKY
CSB-EP461188DKY
CSB-BP461188DKY
CSB-MP461188DKY
CSB-EP461188DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae E3 UFM1-protein ligase 1 homolog (GF17327), partial CSB-YP461189DKY
CSB-EP461189DKY
CSB-BP461189DKY
CSB-MP461189DKY
CSB-EP461189DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Molybdopterin synthase sulfur carrier subunit (Mocs2) CSB-YP461190DKY
CSB-EP461190DKY
CSB-BP461190DKY
CSB-MP461190DKY
CSB-EP461190DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Adenylosuccinate synthetase (GF17012) CSB-YP461191DKY
CSB-EP461191DKY
CSB-BP461191DKY
CSB-MP461191DKY
CSB-EP461191DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial (GF24309), partial CSB-YP461192DKY
CSB-EP461192DKY
CSB-BP461192DKY
CSB-MP461192DKY
CSB-EP461192DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Cytosolic Fe-S cluster assembly factor NUBP2 homolog (GF10354) CSB-YP461193DKY
CSB-EP461193DKY
CSB-BP461193DKY
CSB-MP461193DKY
CSB-EP461193DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Protein CLP1 homolog (cbc) CSB-YP461194DKY
CSB-EP461194DKY
CSB-BP461194DKY
CSB-MP461194DKY
CSB-EP461194DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Regulator of telomere elongation helicase 1 homolog (GF20802), partial CSB-YP461195DKY
CSB-EP461195DKY
CSB-BP461195DKY
CSB-MP461195DKY
CSB-EP461195DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Cytosolic Fe-S cluster assembly factor NUBP1 homolog (GF20875) CSB-YP461196DKY
CSB-EP461196DKY
CSB-BP461196DKY
CSB-MP461196DKY
CSB-EP461196DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Eukaryotic translation initiation factor 3 subunit G-1 (eIF3-S4-1) CSB-YP461197DKY
CSB-EP461197DKY
CSB-BP461197DKY
CSB-MP461197DKY
CSB-EP461197DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein Turandot M (TotM) CSB-YP461198DLK
CSB-EP461198DLK
CSB-BP461198DLK
CSB-MP461198DLK
CSB-EP461198DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Elongation factor G, mitochondrial (ico), partial CSB-YP461199DLK
CSB-EP461199DLK
CSB-BP461199DLK
CSB-MP461199DLK
CSB-EP461199DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Calcium channel flower (flower), partial CSB-YP461200DLK1
CSB-EP461200DLK1
CSB-BP461200DLK1
CSB-MP461200DLK1
CSB-EP461200DLK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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