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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus reuteri 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP462002LMV
CSB-EP462002LMV
CSB-BP462002LMV
CSB-MP462002LMV
CSB-EP462002LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri tRNA (guanine-N (1)-)-methyltransferase CSB-YP462003LMV
CSB-EP462003LMV
CSB-BP462003LMV
CSB-MP462003LMV
CSB-EP462003LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP462004LMV
CSB-EP462004LMV
CSB-BP462004LMV
CSB-MP462004LMV
CSB-EP462004LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP462005LMV
CSB-EP462005LMV
CSB-BP462005LMV
CSB-MP462005LMV
CSB-EP462005LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L33 2 (rpmG2) CSB-YP462006LMV
CSB-EP462006LMV
CSB-BP462006LMV
CSB-MP462006LMV
CSB-EP462006LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri UPF0342 protein LAR_1202 (LAR_1202) CSB-YP462007LMV
CSB-EP462007LMV
CSB-BP462007LMV
CSB-MP462007LMV
CSB-EP462007LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Xanthine phosphoribosyltransferase (xpt) CSB-YP462008LMV
CSB-EP462008LMV
CSB-BP462008LMV
CSB-MP462008LMV
CSB-EP462008LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Adenylate kinase (adk) CSB-YP462009LMV
CSB-EP462009LMV
CSB-BP462009LMV
CSB-MP462009LMV
CSB-EP462009LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L5 (rplE) CSB-YP462010LMV
CSB-EP462010LMV
CSB-BP462010LMV
CSB-MP462010LMV
CSB-EP462010LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 50S ribosomal protein L23 (rplW) CSB-YP462011LMV
CSB-EP462011LMV
CSB-BP462011LMV
CSB-MP462011LMV
CSB-EP462011LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Glutamyl-tRNA reductase (hemA) CSB-YP462012LMV
CSB-EP462012LMV
CSB-BP462012LMV
CSB-MP462012LMV
CSB-EP462012LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP462013LMO
CSB-EP462013LMO
CSB-BP462013LMO
CSB-MP462013LMO
CSB-EP462013LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Glutamate--tRNA ligase (gltX) CSB-YP462014LMO
CSB-EP462014LMO
CSB-BP462014LMO
CSB-MP462014LMO
CSB-EP462014LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum 50S ribosomal protein L1 (rplA) CSB-YP462015LMO
CSB-EP462015LMO
CSB-BP462015LMO
CSB-MP462015LMO
CSB-EP462015LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP462016LMO
CSB-EP462016LMO
CSB-BP462016LMO
CSB-MP462016LMO
CSB-EP462016LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Putative membrane protein insertion efficiency factor (LAF_0446) CSB-YP462017LMO
CSB-EP462017LMO
CSB-BP462017LMO
CSB-MP462017LMO
CSB-EP462017LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum DNA mismatch repair protein MutS (mutS), partial CSB-YP462018LMO
CSB-EP462018LMO
CSB-BP462018LMO
CSB-MP462018LMO
CSB-EP462018LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Probable transcriptional regulatory protein LAF_0541 (LAF_0541) CSB-YP462019LMO
CSB-EP462019LMO
CSB-BP462019LMO
CSB-MP462019LMO
CSB-EP462019LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP462020LMO
CSB-EP462020LMO
CSB-BP462020LMO
CSB-MP462020LMO
CSB-EP462020LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Histidine--tRNA ligase (hisS) CSB-YP462021LMO
CSB-EP462021LMO
CSB-BP462021LMO
CSB-MP462021LMO
CSB-EP462021LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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