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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermoproteus neutrophilus Shikimate dehydrogenase (aroE) CSB-YP461801TNE
CSB-EP461801TNE
CSB-BP461801TNE
CSB-MP461801TNE
CSB-EP461801TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Alanine--tRNA ligase (alaS), partial CSB-YP461802TNE
CSB-EP461802TNE
CSB-BP461802TNE
CSB-MP461802TNE
CSB-EP461802TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Uracil phosphoribosyltransferase (upp) CSB-YP461803EPT
CSB-EP461803EPT
CSB-BP461803EPT
CSB-MP461803EPT
CSB-EP461803EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Ferredoxin--NADP reductase 2 (Exig_2773) CSB-YP461804EPT
CSB-EP461804EPT
CSB-BP461804EPT
CSB-MP461804EPT
CSB-EP461804EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP461805EPT
CSB-EP461805EPT
CSB-BP461805EPT
CSB-MP461805EPT
CSB-EP461805EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP461806EPT
CSB-EP461806EPT
CSB-BP461806EPT
CSB-MP461806EPT
CSB-EP461806EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum UPF0133 protein Exig_0019 (Exig_0019) CSB-YP461807EPT
CSB-EP461807EPT
CSB-BP461807EPT
CSB-MP461807EPT
CSB-EP461807EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Glutamate--tRNA ligase (gltX) CSB-YP461808EPT
CSB-EP461808EPT
CSB-BP461808EPT
CSB-MP461808EPT
CSB-EP461808EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 30S ribosomal protein S10 (rpsJ) CSB-YP461809EPT
CSB-EP461809EPT
CSB-BP461809EPT
CSB-MP461809EPT
CSB-EP461809EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 30S ribosomal protein S3 (rpsC) CSB-YP461810EPT
CSB-EP461810EPT
CSB-BP461810EPT
CSB-MP461810EPT
CSB-EP461810EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L5 (rplE) CSB-YP461811EPT
CSB-EP461811EPT
CSB-BP461811EPT
CSB-MP461811EPT
CSB-EP461811EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 30S ribosomal protein S13 (rpsM) CSB-YP461812EPT
CSB-EP461812EPT
CSB-BP461812EPT
CSB-MP461812EPT
CSB-EP461812EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 30S ribosomal protein S9 (rpsI) CSB-YP461813EPT
CSB-EP461813EPT
CSB-BP461813EPT
CSB-MP461813EPT
CSB-EP461813EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP461814EPT
CSB-EP461814EPT
CSB-BP461814EPT
CSB-MP461814EPT
CSB-EP461814EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Cytidylate kinase (cmk) CSB-YP461815EPT
CSB-EP461815EPT
CSB-BP461815EPT
CSB-MP461815EPT
CSB-EP461815EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 30S ribosomal protein S2 (rpsB) CSB-YP461816EPT
CSB-EP461816EPT
CSB-BP461816EPT
CSB-MP461816EPT
CSB-EP461816EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Ribosome maturation factor RimM (rimM) CSB-YP461817EPT
CSB-EP461817EPT
CSB-BP461817EPT
CSB-MP461817EPT
CSB-EP461817EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum UPF0173 metal-dependent hydrolase Exig_1934 (Exig_1934) CSB-YP461818EPT
CSB-EP461818EPT
CSB-BP461818EPT
CSB-MP461818EPT
CSB-EP461818EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP461819EPT
CSB-EP461819EPT
CSB-BP461819EPT
CSB-MP461819EPT
CSB-EP461819EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Acireductone dioxygenase (mtnD) CSB-YP461820EPT
CSB-EP461820EPT
CSB-BP461820EPT
CSB-MP461820EPT
CSB-EP461820EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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