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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Amoebophilus asiaticus 50S ribosomal protein L33 (rpmG) CSB-YP461101BYI
CSB-EP461101BYI
CSB-BP461101BYI
CSB-MP461101BYI
CSB-EP461101BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus Probable transcriptional regulatory protein Aasi_0624 (Aasi_0624) CSB-YP461102BYI
CSB-EP461102BYI
CSB-BP461102BYI
CSB-MP461102BYI
CSB-EP461102BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus Transcription elongation factor GreA (greA) CSB-YP461103BYI
CSB-EP461103BYI
CSB-BP461103BYI
CSB-MP461103BYI
CSB-EP461103BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus Bifunctional protein FolD (folD) CSB-YP461104BYI
CSB-EP461104BYI
CSB-BP461104BYI
CSB-MP461104BYI
CSB-EP461104BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus tRNA modification GTPase MnmE (mnmE) CSB-YP461105BYI
CSB-EP461105BYI
CSB-BP461105BYI
CSB-MP461105BYI
CSB-EP461105BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP461106BYI
CSB-EP461106BYI
CSB-BP461106BYI
CSB-MP461106BYI
CSB-EP461106BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus 30S ribosomal protein S15 (rpsO) CSB-YP461107BYI
CSB-EP461107BYI
CSB-BP461107BYI
CSB-MP461107BYI
CSB-EP461107BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus 30S ribosomal protein S10 (rpsJ) CSB-YP461108BYI
CSB-EP461108BYI
CSB-BP461108BYI
CSB-MP461108BYI
CSB-EP461108BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus Enolase (eno) CSB-YP461109BYI
CSB-EP461109BYI
CSB-BP461109BYI
CSB-MP461109BYI
CSB-EP461109BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus 30S ribosomal protein S8 (rpsH) CSB-YP461110BYI
CSB-EP461110BYI
CSB-BP461110BYI
CSB-MP461110BYI
CSB-EP461110BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus 50S ribosomal protein L2 (rplB) CSB-YP461111BYI
CSB-EP461111BYI
CSB-BP461111BYI
CSB-MP461111BYI
CSB-EP461111BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP461112IMU
CSB-EP461112IMU
CSB-BP461112IMU
CSB-MP461112IMU
CSB-EP461112IMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Palicourea rigida Parigidin-br1 CSB-YP461113EQS
CSB-EP461113EQS
CSB-BP461113EQS
CSB-MP461113EQS
CSB-EP461113EQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM25B (FAM25B) CSB-YP461114HU
CSB-EP461114HU
CSB-BP461114HU
CSB-MP461114HU
CSB-EP461114HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus vitulinus Conotoxin Vt15.1 CSB-YP461115DWN
CSB-EP461115DWN
CSB-BP461115DWN
CSB-MP461115DWN
CSB-EP461115DWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti Lectin SHL-Ib2 CSB-YP461116HUL
CSB-EP461116HUL
CSB-BP461116HUL
CSB-MP461116HUL
CSB-EP461116HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Tryptophan synthase beta chain (trpB) CSB-YP461117AXI
CSB-EP461117AXI
CSB-BP461117AXI
CSB-MP461117AXI
CSB-EP461117AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP461118AXI
CSB-EP461118AXI
CSB-BP461118AXI
CSB-MP461118AXI
CSB-EP461118AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Probable rRNA maturation factor (APP7_0736) CSB-YP461119AXI
CSB-EP461119AXI
CSB-BP461119AXI
CSB-MP461119AXI
CSB-EP461119AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Integration host factor subunit beta (ihfB) CSB-YP461120AXI
CSB-EP461120AXI
CSB-BP461120AXI
CSB-MP461120AXI
CSB-EP461120AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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