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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Guizotia abyssinica NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP460441GHI1
CSB-EP460441GHI1
CSB-BP460441GHI1
CSB-MP460441GHI1
CSB-EP460441GHI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parana virus RNA-directed RNA polymerase L (L), partial CSB-YP460442ERX
CSB-EP460442ERX
CSB-BP460442ERX
CSB-MP460442ERX
CSB-EP460442ERX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L19 (rplS) CSB-YP460443EXZ
CSB-EP460443EXZ
CSB-BP460443EXZ
CSB-MP460443EXZ
CSB-EP460443EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP460444EXZ
CSB-EP460444EXZ
CSB-BP460444EXZ
CSB-MP460444EXZ
CSB-EP460444EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Putative membrane protein insertion efficiency factor (PGN_0308) CSB-YP460445EXZ
CSB-EP460445EXZ
CSB-BP460445EXZ
CSB-MP460445EXZ
CSB-EP460445EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 30S ribosomal protein S20 (rpsT) CSB-YP460446EXZ
CSB-EP460446EXZ
CSB-BP460446EXZ
CSB-MP460446EXZ
CSB-EP460446EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Undecaprenyl-diphosphatase (uppP), partial CSB-YP460447EXZ
CSB-EP460447EXZ
CSB-BP460447EXZ
CSB-MP460447EXZ
CSB-EP460447EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP460448EXZ
CSB-EP460448EXZ
CSB-BP460448EXZ
CSB-MP460448EXZ
CSB-EP460448EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Lon protease (lon), partial CSB-YP460449EXZ
CSB-EP460449EXZ
CSB-BP460449EXZ
CSB-MP460449EXZ
CSB-EP460449EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis D-alanine--D-alanine ligase (ddl) CSB-YP460450EXZ
CSB-EP460450EXZ
CSB-BP460450EXZ
CSB-MP460450EXZ
CSB-EP460450EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis GTPase obg (obg) CSB-YP460451EXZ
CSB-EP460451EXZ
CSB-BP460451EXZ
CSB-MP460451EXZ
CSB-EP460451EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L35 (rpmI) CSB-YP460452EXZ
CSB-EP460452EXZ
CSB-BP460452EXZ
CSB-MP460452EXZ
CSB-EP460452EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Hydroxylamine reductase (hcp), partial CSB-YP460453EXZ
CSB-EP460453EXZ
CSB-BP460453EXZ
CSB-MP460453EXZ
CSB-EP460453EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP460454EXZ
CSB-EP460454EXZ
CSB-BP460454EXZ
CSB-MP460454EXZ
CSB-EP460454EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP460455EXZ
CSB-EP460455EXZ
CSB-BP460455EXZ
CSB-MP460455EXZ
CSB-EP460455EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 10 kDa chaperonin (groS) CSB-YP460456EXZ
CSB-EP460456EXZ
CSB-BP460456EXZ
CSB-MP460456EXZ
CSB-EP460456EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Type III pantothenate kinase (coaX) CSB-YP460457EXZ
CSB-EP460457EXZ
CSB-BP460457EXZ
CSB-MP460457EXZ
CSB-EP460457EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L7/L12 (rplL) CSB-YP460458EXZ
CSB-EP460458EXZ
CSB-BP460458EXZ
CSB-MP460458EXZ
CSB-EP460458EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Probable GTP-binding protein EngB (engB) CSB-YP460459EXZ
CSB-EP460459EXZ
CSB-BP460459EXZ
CSB-MP460459EXZ
CSB-EP460459EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Fructose-bisphosphate aldolase class 1 (fda) CSB-YP460460EXZ
CSB-EP460460EXZ
CSB-BP460460EXZ
CSB-MP460460EXZ
CSB-EP460460EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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