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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechococcus sp. Glutamyl-tRNA reductase (hemA) CSB-YP461721FQA
CSB-EP461721FQA
CSB-BP461721FQA
CSB-MP461721FQA
CSB-EP461721FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Bifunctional protein GlmU (glmU) CSB-YP461722FQA
CSB-EP461722FQA
CSB-BP461722FQA
CSB-MP461722FQA
CSB-EP461722FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. D-tyrosyl-tRNA (Tyr) deacylase CSB-YP461723FQA
CSB-EP461723FQA
CSB-BP461723FQA
CSB-MP461723FQA
CSB-EP461723FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Putative chromophore lyase CpcV (cpcV) CSB-YP461724FQA
CSB-EP461724FQA
CSB-BP461724FQA
CSB-MP461724FQA
CSB-EP461724FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Proton extrusion protein PcxA (pcxA), partial CSB-YP461725FQA1
CSB-EP461725FQA1
CSB-BP461725FQA1
CSB-MP461725FQA1
CSB-EP461725FQA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP461726FQA
CSB-EP461726FQA
CSB-BP461726FQA
CSB-MP461726FQA
CSB-EP461726FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoglycerate kinase (pgk) CSB-YP461727FQA
CSB-EP461727FQA
CSB-BP461727FQA
CSB-MP461727FQA
CSB-EP461727FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Diaminopimelate epimerase (dapF) CSB-YP461728FQA
CSB-EP461728FQA
CSB-BP461728FQA
CSB-MP461728FQA
CSB-EP461728FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Bifunctional protein pyrR (pyrR) CSB-YP461729FQA
CSB-EP461729FQA
CSB-BP461729FQA
CSB-MP461729FQA
CSB-EP461729FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP461730FQA
CSB-EP461730FQA
CSB-BP461730FQA
CSB-MP461730FQA
CSB-EP461730FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Acireductone dioxygenase (mtnD) CSB-YP461731FQA
CSB-EP461731FQA
CSB-BP461731FQA
CSB-MP461731FQA
CSB-EP461731FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP phosphoribosyltransferase (hisG) CSB-YP461732FQA
CSB-EP461732FQA
CSB-BP461732FQA
CSB-MP461732FQA
CSB-EP461732FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal protein S6 modification protein (rimK) CSB-YP461733FQA
CSB-EP461733FQA
CSB-BP461733FQA
CSB-MP461733FQA
CSB-EP461733FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Allophycocyanin alpha subunit (apcA) CSB-YP461734FQA
CSB-EP461734FQA
CSB-BP461734FQA
CSB-MP461734FQA
CSB-EP461734FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP461735EXM
CSB-EP461735EXM
CSB-BP461735EXM
CSB-MP461735EXM
CSB-EP461735EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP461736EXM
CSB-EP461736EXM
CSB-BP461736EXM
CSB-MP461736EXM
CSB-EP461736EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP461737EXM
CSB-EP461737EXM
CSB-BP461737EXM
CSB-MP461737EXM
CSB-EP461737EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Arginine--tRNA ligase (argS), partial CSB-YP461738EXM
CSB-EP461738EXM
CSB-BP461738EXM
CSB-MP461738EXM
CSB-EP461738EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Membrane protein insertase YidC (yidC), partial CSB-YP461739EXM1
CSB-EP461739EXM1
CSB-BP461739EXM1
CSB-MP461739EXM1
CSB-EP461739EXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L29 (rpmC) CSB-YP461740EXM
CSB-EP461740EXM
CSB-BP461740EXM
CSB-MP461740EXM
CSB-EP461740EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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