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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neisseria gonorrhoeae Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP461661NGB
CSB-EP461661NGB
CSB-BP461661NGB
CSB-MP461661NGB
CSB-EP461661NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Cell division protein FtsB (ftsB), partial CSB-YP461662NGB
CSB-EP461662NGB
CSB-BP461662NGB
CSB-MP461662NGB
CSB-EP461662NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Glutamyl-tRNA reductase (hemA) CSB-YP461663NGB
CSB-EP461663NGB
CSB-BP461663NGB
CSB-MP461663NGB
CSB-EP461663NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Probable intracellular septation protein A (NGK_2044), partial CSB-YP461664NGB1
CSB-EP461664NGB1
CSB-BP461664NGB1
CSB-MP461664NGB1
CSB-EP461664NGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP461665NGB
CSB-EP461665NGB
CSB-BP461665NGB
CSB-MP461665NGB
CSB-EP461665NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP461666NGB
CSB-EP461666NGB
CSB-BP461666NGB
CSB-MP461666NGB
CSB-EP461666NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP461667NGB
CSB-EP461667NGB
CSB-BP461667NGB
CSB-MP461667NGB
CSB-EP461667NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Probable GTP-binding protein EngB (engB) CSB-YP461668NGB
CSB-EP461668NGB
CSB-BP461668NGB
CSB-MP461668NGB
CSB-EP461668NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Lipid II flippase FtsW (ftsW), partial CSB-YP461669NGB
CSB-EP461669NGB
CSB-BP461669NGB
CSB-MP461669NGB
CSB-EP461669NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 30S ribosomal protein S9 (rpsI) CSB-YP461670NGB
CSB-EP461670NGB
CSB-BP461670NGB
CSB-MP461670NGB
CSB-EP461670NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae 50S ribosomal protein L29 (rpmC) CSB-YP461671NGB
CSB-EP461671NGB
CSB-BP461671NGB
CSB-MP461671NGB
CSB-EP461671NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae tRNA modification GTPase MnmE (mnmE) CSB-YP461672NGB
CSB-EP461672NGB
CSB-BP461672NGB
CSB-MP461672NGB
CSB-EP461672NGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii ATP synthase gamma chain (atpG) CSB-YP461673AZX
CSB-EP461673AZX
CSB-BP461673AZX
CSB-MP461673AZX
CSB-EP461673AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L20 (rplT) CSB-YP461674AZX
CSB-EP461674AZX
CSB-BP461674AZX
CSB-MP461674AZX
CSB-EP461674AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP461675AZX
CSB-EP461675AZX
CSB-BP461675AZX
CSB-MP461675AZX
CSB-EP461675AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L24 (rplX) CSB-YP461676AZX
CSB-EP461676AZX
CSB-BP461676AZX
CSB-MP461676AZX
CSB-EP461676AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L15 (rplO) CSB-YP461677AZX
CSB-EP461677AZX
CSB-BP461677AZX
CSB-MP461677AZX
CSB-EP461677AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 7-cyano-7-deazaguanine synthase (queC) CSB-YP461678AZX
CSB-EP461678AZX
CSB-BP461678AZX
CSB-MP461678AZX
CSB-EP461678AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Adenylate kinase (adk) CSB-YP461679AZX
CSB-EP461679AZX
CSB-BP461679AZX
CSB-MP461679AZX
CSB-EP461679AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP461680AZX
CSB-EP461680AZX
CSB-BP461680AZX
CSB-MP461680AZX
CSB-EP461680AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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