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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 Probable RNA 2'-phosphotransferase (kptA) CSB-YP462642SYZ
CSB-EP462642SYZ
CSB-BP462642SYZ
CSB-MP462642SYZ
CSB-EP462642SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP462643SYZ
CSB-EP462643SYZ
CSB-BP462643SYZ
CSB-MP462643SYZ
CSB-EP462643SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP462644SYZ
CSB-EP462644SYZ
CSB-BP462644SYZ
CSB-MP462644SYZ
CSB-EP462644SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP462645SYZ
CSB-EP462645SYZ
CSB-BP462645SYZ
CSB-MP462645SYZ
CSB-EP462645SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 tRNA pseudouridine synthase D (truD) CSB-YP462646SYZ
CSB-EP462646SYZ
CSB-BP462646SYZ
CSB-MP462646SYZ
CSB-EP462646SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Peptide chain release factor 3 (prfC), partial CSB-YP462647SYZ
CSB-EP462647SYZ
CSB-BP462647SYZ
CSB-MP462647SYZ
CSB-EP462647SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP462648SYZ
CSB-EP462648SYZ
CSB-BP462648SYZ
CSB-MP462648SYZ
CSB-EP462648SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0225 protein ychJ (ychJ) CSB-YP462649SYZ
CSB-EP462649SYZ
CSB-BP462649SYZ
CSB-MP462649SYZ
CSB-EP462649SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein RecA (recA) CSB-YP462650SYZ
CSB-EP462650SYZ
CSB-BP462650SYZ
CSB-MP462650SYZ
CSB-EP462650SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein CsiD (csiD) CSB-YP462651SYZ
CSB-EP462651SYZ
CSB-BP462651SYZ
CSB-MP462651SYZ
CSB-EP462651SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP462652SYZ
CSB-EP462652SYZ
CSB-BP462652SYZ
CSB-MP462652SYZ
CSB-EP462652SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 S-adenosylmethionine synthase (metK) CSB-YP462653SYZ
CSB-EP462653SYZ
CSB-BP462653SYZ
CSB-MP462653SYZ
CSB-EP462653SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP462654SYZ
CSB-EP462654SYZ
CSB-BP462654SYZ
CSB-MP462654SYZ
CSB-EP462654SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP462655SYZ1
CSB-EP462655SYZ1
CSB-BP462655SYZ1
CSB-MP462655SYZ1
CSB-EP462655SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP462656SYZ
CSB-EP462656SYZ
CSB-BP462656SYZ
CSB-MP462656SYZ
CSB-EP462656SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 GTPase obg (obg) CSB-YP462657SYZ
CSB-EP462657SYZ
CSB-BP462657SYZ
CSB-MP462657SYZ
CSB-EP462657SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP462658SYZ
CSB-EP462658SYZ
CSB-BP462658SYZ
CSB-MP462658SYZ
CSB-EP462658SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP462659SYZ
CSB-EP462659SYZ
CSB-BP462659SYZ
CSB-MP462659SYZ
CSB-EP462659SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP462660SYZ
CSB-EP462660SYZ
CSB-BP462660SYZ
CSB-MP462660SYZ
CSB-EP462660SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP462661SYZ
CSB-EP462661SYZ
CSB-BP462661SYZ
CSB-MP462661SYZ
CSB-EP462661SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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