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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methylacidiphilum infernorum UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP464902MSV
CSB-EP464902MSV
CSB-BP464902MSV
CSB-MP464902MSV
CSB-EP464902MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 33 kDa chaperonin (hslO) CSB-YP464903GFO
CSB-EP464903GFO
CSB-BP464903GFO
CSB-MP464903GFO
CSB-EP464903GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP464904GFO
CSB-EP464904GFO
CSB-BP464904GFO
CSB-MP464904GFO
CSB-EP464904GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Probable GTP-binding protein EngB (engB) CSB-YP464905GFO
CSB-EP464905GFO
CSB-BP464905GFO
CSB-MP464905GFO
CSB-EP464905GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP464906GFO
CSB-EP464906GFO
CSB-BP464906GFO
CSB-MP464906GFO
CSB-EP464906GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Undecaprenyl-diphosphatase (uppP), partial CSB-YP464907GFO1
CSB-EP464907GFO1
CSB-BP464907GFO1
CSB-MP464907GFO1
CSB-EP464907GFO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Dihydroorotase (pyrC) CSB-YP464908GFO
CSB-EP464908GFO
CSB-BP464908GFO
CSB-MP464908GFO
CSB-EP464908GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Membrane protein insertase YidC (yidC), partial CSB-YP464909GFO1
CSB-EP464909GFO1
CSB-BP464909GFO1
CSB-MP464909GFO1
CSB-EP464909GFO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Protein MraZ (mraZ) CSB-YP464910GFO
CSB-EP464910GFO
CSB-BP464910GFO
CSB-MP464910GFO
CSB-EP464910GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP464911GFO
CSB-EP464911GFO
CSB-BP464911GFO
CSB-MP464911GFO
CSB-EP464911GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi NH (3)-dependent NAD (+) synthetase CSB-YP464912GFO
CSB-EP464912GFO
CSB-BP464912GFO
CSB-MP464912GFO
CSB-EP464912GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP464913GFO
CSB-EP464913GFO
CSB-BP464913GFO
CSB-MP464913GFO
CSB-EP464913GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Protein translocase subunit SecA (secA), partial CSB-YP464914GFO
CSB-EP464914GFO
CSB-BP464914GFO
CSB-MP464914GFO
CSB-EP464914GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi GTPase obg (obg) CSB-YP464915GFO
CSB-EP464915GFO
CSB-BP464915GFO
CSB-MP464915GFO
CSB-EP464915GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP464916GFO
CSB-EP464916GFO
CSB-BP464916GFO
CSB-MP464916GFO
CSB-EP464916GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP464917GFO
CSB-EP464917GFO
CSB-BP464917GFO
CSB-MP464917GFO
CSB-EP464917GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 30S ribosomal protein S18 (rpsR) CSB-YP464918GFO
CSB-EP464918GFO
CSB-BP464918GFO
CSB-MP464918GFO
CSB-EP464918GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi Type III pantothenate kinase (coaX) CSB-YP464919GFO
CSB-EP464919GFO
CSB-BP464919GFO
CSB-MP464919GFO
CSB-EP464919GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP464920GFO
CSB-EP464920GFO
CSB-BP464920GFO
CSB-MP464920GFO
CSB-EP464920GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi 30S ribosomal protein S3 (rpsC) CSB-YP464921GFO
CSB-EP464921GFO
CSB-BP464921GFO
CSB-MP464921GFO
CSB-EP464921GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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