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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Alteromonas macleodii UPF0434 protein MADE_1009415 (MADE_1009415) CSB-YP463622AZX
CSB-EP463622AZX
CSB-BP463622AZX
CSB-MP463622AZX
CSB-EP463622AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP463623AZX
CSB-EP463623AZX
CSB-BP463623AZX
CSB-MP463623AZX
CSB-EP463623AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP463624ENT
CSB-EP463624ENT
CSB-BP463624ENT
CSB-MP463624ENT
CSB-EP463624ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0133 protein ybaB (ybaB) CSB-YP463625ENT
CSB-EP463625ENT
CSB-BP463625ENT
CSB-MP463625ENT
CSB-EP463625ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Tyrosine--tRNA ligase (tyrS) CSB-YP463626ENT
CSB-EP463626ENT
CSB-BP463626ENT
CSB-MP463626ENT
CSB-EP463626ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0061 protein ydiU (ydiU) CSB-YP463627ENT
CSB-EP463627ENT
CSB-BP463627ENT
CSB-MP463627ENT
CSB-EP463627ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycogen synthesis protein glgS (glgS) CSB-YP463628ENT
CSB-EP463628ENT
CSB-BP463628ENT
CSB-MP463628ENT
CSB-EP463628ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S21 (rpsU) CSB-YP463629ENT
CSB-EP463629ENT
CSB-BP463629ENT
CSB-MP463629ENT
CSB-EP463629ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Bifunctional protein FolD (folD) CSB-YP463630ENT
CSB-EP463630ENT
CSB-BP463630ENT
CSB-MP463630ENT
CSB-EP463630ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP463631ENT
CSB-EP463631ENT
CSB-BP463631ENT
CSB-MP463631ENT
CSB-EP463631ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Threonine/serine transporter TdcC (tdcC), partial CSB-YP463632ENT
CSB-EP463632ENT
CSB-BP463632ENT
CSB-MP463632ENT
CSB-EP463632ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0213 protein yhbQ (yhbQ) CSB-YP463633ENT
CSB-EP463633ENT
CSB-BP463633ENT
CSB-MP463633ENT
CSB-EP463633ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protease HtpX (htpX), partial CSB-YP463634ENT1
CSB-EP463634ENT1
CSB-BP463634ENT1
CSB-MP463634ENT1
CSB-EP463634ENT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA (mo5U34)-methyltransferase CSB-YP463635ENT
CSB-EP463635ENT
CSB-BP463635ENT
CSB-MP463635ENT
CSB-EP463635ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP463636ENT
CSB-EP463636ENT
CSB-BP463636ENT
CSB-MP463636ENT
CSB-EP463636ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L13 (rplM) CSB-YP463637ENT
CSB-EP463637ENT
CSB-BP463637ENT
CSB-MP463637ENT
CSB-EP463637ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UPF0234 protein SYNPCC7002_A1983 (SYNPCC7002_A1983) CSB-YP463638FQA
CSB-EP463638FQA
CSB-BP463638FQA
CSB-MP463638FQA
CSB-EP463638FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase subunit c (atpE), partial CSB-YP463639FQA1
CSB-EP463639FQA1
CSB-BP463639FQA1
CSB-MP463639FQA1
CSB-EP463639FQA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP463640FQA
CSB-EP463640FQA
CSB-BP463640FQA
CSB-MP463640FQA
CSB-EP463640FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Dihydrodipicolinate reductase (dapB) CSB-YP463641FQA
CSB-EP463641FQA
CSB-BP463641FQA
CSB-MP463641FQA
CSB-EP463641FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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