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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium marinum 30S ribosomal protein S12 (rpsL) CSB-YP464022MVS
CSB-EP464022MVS
CSB-BP464022MVS
CSB-MP464022MVS
CSB-EP464022MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L4 (rplD) CSB-YP464023MVS
CSB-EP464023MVS
CSB-BP464023MVS
CSB-MP464023MVS
CSB-EP464023MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L29 (rpmC) CSB-YP464024MVS
CSB-EP464024MVS
CSB-BP464024MVS
CSB-MP464024MVS
CSB-EP464024MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Urease accessory protein UreG (ureG) CSB-YP464025MVS
CSB-EP464025MVS
CSB-BP464025MVS
CSB-MP464025MVS
CSB-EP464025MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP464026AWO
CSB-EP464026AWO
CSB-BP464026AWO
CSB-MP464026AWO
CSB-EP464026AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Histidinol-phosphate aminotransferase (hisC) CSB-YP464027AWO
CSB-EP464027AWO
CSB-BP464027AWO
CSB-MP464027AWO
CSB-EP464027AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Uracil phosphoribosyltransferase (upp) CSB-YP464028AWO
CSB-EP464028AWO
CSB-BP464028AWO
CSB-MP464028AWO
CSB-EP464028AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Type III pantothenate kinase (coaX) CSB-YP464029AWO
CSB-EP464029AWO
CSB-BP464029AWO
CSB-MP464029AWO
CSB-EP464029AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Phosphopantetheine adenylyltransferase (coaD) CSB-YP464030AWO
CSB-EP464030AWO
CSB-BP464030AWO
CSB-MP464030AWO
CSB-EP464030AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable malate:quinone oxidoreductase (mqo), partial CSB-YP464031AWO
CSB-EP464031AWO
CSB-BP464031AWO
CSB-MP464031AWO
CSB-EP464031AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable D-serine dehydratase (dsdA) CSB-YP464032AWO
CSB-EP464032AWO
CSB-BP464032AWO
CSB-MP464032AWO
CSB-EP464032AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP464033AWO
CSB-EP464033AWO
CSB-BP464033AWO
CSB-MP464033AWO
CSB-EP464033AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP464034AWO
CSB-EP464034AWO
CSB-BP464034AWO
CSB-MP464034AWO
CSB-EP464034AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Glutamate 5-kinase (proB) CSB-YP464035AWO
CSB-EP464035AWO
CSB-BP464035AWO
CSB-MP464035AWO
CSB-EP464035AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP464036AWO
CSB-EP464036AWO
CSB-BP464036AWO
CSB-MP464036AWO
CSB-EP464036AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii DNA mismatch repair protein MutS (mutS), partial CSB-YP464037AWO
CSB-EP464037AWO
CSB-BP464037AWO
CSB-MP464037AWO
CSB-EP464037AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii UPF0271 protein ACICU_01267 (ACICU_01267) CSB-YP464038AWO
CSB-EP464038AWO
CSB-BP464038AWO
CSB-MP464038AWO
CSB-EP464038AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Proline--tRNA ligase (proS), partial CSB-YP464039AWO
CSB-EP464039AWO
CSB-BP464039AWO
CSB-MP464039AWO
CSB-EP464039AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP464040AWO
CSB-EP464040AWO
CSB-BP464040AWO
CSB-MP464040AWO
CSB-EP464040AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L13 (rplM) CSB-YP464041AWO
CSB-EP464041AWO
CSB-BP464041AWO
CSB-MP464041AWO
CSB-EP464041AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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