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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Ralstonia pickettii Acetaldehyde dehydrogenase (Rpic_4618) CSB-YP464662RJI
CSB-EP464662RJI
CSB-BP464662RJI
CSB-MP464662RJI
CSB-EP464662RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Chaperone protein DnaK (dnaK), partial CSB-YP464663AZF
CSB-EP464663AZF
CSB-BP464663AZF
CSB-MP464663AZF
CSB-EP464663AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 50S ribosomal protein L17 (rplQ) CSB-YP464664AZF
CSB-EP464664AZF
CSB-BP464664AZF
CSB-MP464664AZF
CSB-EP464664AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila DNA mismatch repair protein mutL (mutL), partial CSB-YP464665AZF
CSB-EP464665AZF
CSB-BP464665AZF
CSB-MP464665AZF
CSB-EP464665AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Bifunctional protein FolD (folD) CSB-YP464666AZF
CSB-EP464666AZF
CSB-BP464666AZF
CSB-MP464666AZF
CSB-EP464666AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 50S ribosomal protein L14 (rplN) CSB-YP464667AZF
CSB-EP464667AZF
CSB-BP464667AZF
CSB-MP464667AZF
CSB-EP464667AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila GTPase Der (der) CSB-YP464668AZF
CSB-EP464668AZF
CSB-BP464668AZF
CSB-MP464668AZF
CSB-EP464668AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP464669AZF
CSB-EP464669AZF
CSB-BP464669AZF
CSB-MP464669AZF
CSB-EP464669AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP464670AZF
CSB-EP464670AZF
CSB-BP464670AZF
CSB-MP464670AZF
CSB-EP464670AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Alpha-1,3-galactosidase B (glaB), partial CSB-YP464671AZF
CSB-EP464671AZF
CSB-BP464671AZF
CSB-MP464671AZF
CSB-EP464671AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Maf-like protein Amuc_0586 (Amuc_0586) CSB-YP464672AZF
CSB-EP464672AZF
CSB-BP464672AZF
CSB-MP464672AZF
CSB-EP464672AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Peptidyl-tRNA hydrolase (pth) CSB-YP464673AZF
CSB-EP464673AZF
CSB-BP464673AZF
CSB-MP464673AZF
CSB-EP464673AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Transcriptional repressor NrdR (nrdR) CSB-YP464674AZF
CSB-EP464674AZF
CSB-BP464674AZF
CSB-MP464674AZF
CSB-EP464674AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 50S ribosomal protein L7/L12 (rplL) CSB-YP464675AZF
CSB-EP464675AZF
CSB-BP464675AZF
CSB-MP464675AZF
CSB-EP464675AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Dihydroxy-acid dehydratase (ilvD), partial CSB-YP464676AZF
CSB-EP464676AZF
CSB-BP464676AZF
CSB-MP464676AZF
CSB-EP464676AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP464677AZF
CSB-EP464677AZF
CSB-BP464677AZF
CSB-MP464677AZF
CSB-EP464677AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S9 (rpsI) CSB-YP464678HUZ
CSB-EP464678HUZ
CSB-BP464678HUZ
CSB-MP464678HUZ
CSB-EP464678HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L20 (rplT) CSB-YP464679HUZ
CSB-EP464679HUZ
CSB-BP464679HUZ
CSB-MP464679HUZ
CSB-EP464679HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Elongation factor P (efp) CSB-YP464680HUZ
CSB-EP464680HUZ
CSB-BP464680HUZ
CSB-MP464680HUZ
CSB-EP464680HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP464681HUZ
CSB-EP464681HUZ
CSB-BP464681HUZ
CSB-MP464681HUZ
CSB-EP464681HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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