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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Orientia tsutsugamushi Ferredoxin--NADP reductase (OTT_1322) CSB-YP464862OEV
CSB-EP464862OEV
CSB-BP464862OEV
CSB-MP464862OEV
CSB-EP464862OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP464863OEV
CSB-EP464863OEV
CSB-BP464863OEV
CSB-MP464863OEV
CSB-EP464863OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L34 (rpmH) CSB-YP464864OEV
CSB-EP464864OEV
CSB-BP464864OEV
CSB-MP464864OEV
CSB-EP464864OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP464865OEV
CSB-EP464865OEV
CSB-BP464865OEV
CSB-MP464865OEV
CSB-EP464865OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP464866OEV
CSB-EP464866OEV
CSB-BP464866OEV
CSB-MP464866OEV
CSB-EP464866OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcystis aeruginosa 50S ribosomal protein L32 (rpmF) CSB-YP464867MTQ
CSB-EP464867MTQ
CSB-BP464867MTQ
CSB-MP464867MTQ
CSB-EP464867MTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0631 protein C17orf108 homolog (zgc:193694) CSB-YP464868DIL
CSB-EP464868DIL
CSB-BP464868DIL
CSB-MP464868DIL
CSB-EP464868DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein shisa-9B (shisa9b), partial CSB-YP464869DIL
CSB-EP464869DIL
CSB-BP464869DIL
CSB-MP464869DIL
CSB-EP464869DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP464870BTA
CSB-EP464870BTA
CSB-BP464870BTA
CSB-MP464870BTA
CSB-EP464870BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP464871BTA
CSB-EP464871BTA
CSB-BP464871BTA
CSB-MP464871BTA
CSB-EP464871BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Arginine--tRNA ligase (argS), partial CSB-YP464872BTA
CSB-EP464872BTA
CSB-BP464872BTA
CSB-MP464872BTA
CSB-EP464872BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L1 (rplA) CSB-YP464873BTA
CSB-EP464873BTA
CSB-BP464873BTA
CSB-MP464873BTA
CSB-EP464873BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 10 kDa chaperonin (groS) CSB-YP464874BTA
CSB-EP464874BTA
CSB-BP464874BTA
CSB-MP464874BTA
CSB-EP464874BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L2 (rplB) CSB-YP464875BTA
CSB-EP464875BTA
CSB-BP464875BTA
CSB-MP464875BTA
CSB-EP464875BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP464876BTA
CSB-EP464876BTA
CSB-BP464876BTA
CSB-MP464876BTA
CSB-EP464876BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP464877BTA
CSB-EP464877BTA
CSB-BP464877BTA
CSB-MP464877BTA
CSB-EP464877BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 30S ribosomal protein S17 (rpsQ) CSB-YP464878BTA
CSB-EP464878BTA
CSB-BP464878BTA
CSB-MP464878BTA
CSB-EP464878BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L18 (rplR) CSB-YP464879BTA
CSB-EP464879BTA
CSB-BP464879BTA
CSB-MP464879BTA
CSB-EP464879BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum 50S ribosomal protein L17 (rplQ) CSB-YP464880BTA
CSB-EP464880BTA
CSB-BP464880BTA
CSB-MP464880BTA
CSB-EP464880BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP464881BTA
CSB-EP464881BTA
CSB-BP464881BTA
CSB-MP464881BTA
CSB-EP464881BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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