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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella enteritidis PT4 Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP466443SWO
CSB-EP466443SWO
CSB-BP466443SWO
CSB-MP466443SWO
CSB-EP466443SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 DNA gyrase inhibitor YacG (yacG) CSB-YP466444SWO
CSB-EP466444SWO
CSB-BP466444SWO
CSB-MP466444SWO
CSB-EP466444SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0266 membrane protein yobD (yobD), partial CSB-YP466445SWO1
CSB-EP466445SWO1
CSB-BP466445SWO1
CSB-MP466445SWO1
CSB-EP466445SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0260 protein ycgN (ycgN) CSB-YP466446SWO
CSB-EP466446SWO
CSB-BP466446SWO
CSB-MP466446SWO
CSB-EP466446SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Endo-type membrane-bound lytic murein transglycosylase A (emtA) CSB-YP466447SWO
CSB-EP466447SWO
CSB-BP466447SWO
CSB-MP466447SWO
CSB-EP466447SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP466448SWO
CSB-EP466448SWO
CSB-BP466448SWO
CSB-MP466448SWO
CSB-EP466448SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0208 membrane protein YfbV (yfbV) CSB-YP466449SWO
CSB-EP466449SWO
CSB-BP466449SWO
CSB-MP466449SWO
CSB-EP466449SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 HTH-type transcriptional regulator malT (malT), partial CSB-YP466450SWO
CSB-EP466450SWO
CSB-BP466450SWO
CSB-MP466450SWO
CSB-EP466450SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Aspartate 1-decarboxylase (panD) CSB-YP466451SWO
CSB-EP466451SWO
CSB-BP466451SWO
CSB-MP466451SWO
CSB-EP466451SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Sugar fermentation stimulation protein A (sfsA) CSB-YP466452SWO
CSB-EP466452SWO
CSB-BP466452SWO
CSB-MP466452SWO
CSB-EP466452SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP466453SWO
CSB-EP466453SWO
CSB-BP466453SWO
CSB-MP466453SWO
CSB-EP466453SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP466454SWO
CSB-EP466454SWO
CSB-BP466454SWO
CSB-MP466454SWO
CSB-EP466454SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP466455SWO
CSB-EP466455SWO
CSB-BP466455SWO
CSB-MP466455SWO
CSB-EP466455SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Lipid-A-disaccharide synthase (lpxB) CSB-YP466456SWO
CSB-EP466456SWO
CSB-BP466456SWO
CSB-MP466456SWO
CSB-EP466456SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Zinc transport protein ZntB (zntB), partial CSB-YP466457SWO1
CSB-EP466457SWO1
CSB-BP466457SWO1
CSB-MP466457SWO1
CSB-EP466457SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Ethanolamine ammonia-lyase light chain (eutC) CSB-YP466458SWO
CSB-EP466458SWO
CSB-BP466458SWO
CSB-MP466458SWO
CSB-EP466458SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Sigma factor-binding protein crl (crl) CSB-YP466459SWO
CSB-EP466459SWO
CSB-BP466459SWO
CSB-MP466459SWO
CSB-EP466459SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP466460SWO
CSB-EP466460SWO
CSB-BP466460SWO
CSB-MP466460SWO
CSB-EP466460SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP466461SWO
CSB-EP466461SWO
CSB-BP466461SWO
CSB-MP466461SWO
CSB-EP466461SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 L-seryl-tRNA (Sec) selenium transferase CSB-YP466462SWO
CSB-EP466462SWO
CSB-BP466462SWO
CSB-MP466462SWO
CSB-EP466462SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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