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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella enteritidis PT4 Nucleoid occlusion factor SlmA (slmA) CSB-YP466463SWO
CSB-EP466463SWO
CSB-BP466463SWO
CSB-MP466463SWO
CSB-EP466463SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0246 protein yaaA (yaaA) CSB-YP466464SWO
CSB-EP466464SWO
CSB-BP466464SWO
CSB-MP466464SWO
CSB-EP466464SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ribonuclease HII (rnhB) CSB-YP466465SWP
CSB-EP466465SWP
CSB-BP466465SWP
CSB-MP466465SWP
CSB-EP466465SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Sigma factor-binding protein crl (crl) CSB-YP466466SWP
CSB-EP466466SWP
CSB-BP466466SWP
CSB-MP466466SWP
CSB-EP466466SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Adenylate kinase (adk) CSB-YP466467SWP
CSB-EP466467SWP
CSB-BP466467SWP
CSB-MP466467SWP
CSB-EP466467SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Glutamine--tRNA ligase (glnS), partial CSB-YP466468SWP
CSB-EP466468SWP
CSB-BP466468SWP
CSB-MP466468SWP
CSB-EP466468SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Endonuclease 8 (nei) CSB-YP466469SWP
CSB-EP466469SWP
CSB-BP466469SWP
CSB-MP466469SWP
CSB-EP466469SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Macrodomain Ter protein (matP) CSB-YP466470SWP
CSB-EP466470SWP
CSB-BP466470SWP
CSB-MP466470SWP
CSB-EP466470SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Tryptophan synthase beta chain (trpB) CSB-YP466471SWP
CSB-EP466471SWP
CSB-BP466471SWP
CSB-MP466471SWP
CSB-EP466471SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum N utilization substance protein B homolog (nusB) CSB-YP466472SWP
CSB-EP466472SWP
CSB-BP466472SWP
CSB-MP466472SWP
CSB-EP466472SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Carboxylate-amine ligase YbdK (ybdK) CSB-YP466473SWP
CSB-EP466473SWP
CSB-BP466473SWP
CSB-MP466473SWP
CSB-EP466473SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Biotin synthase (bioB) CSB-YP466474SWP
CSB-EP466474SWP
CSB-BP466474SWP
CSB-MP466474SWP
CSB-EP466474SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Flagellar hook-basal body complex protein FliE (fliE) CSB-YP466475SWP
CSB-EP466475SWP
CSB-BP466475SWP
CSB-MP466475SWP
CSB-EP466475SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Siroheme synthase (cysG) CSB-YP466476SWP
CSB-EP466476SWP
CSB-BP466476SWP
CSB-MP466476SWP
CSB-EP466476SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP466477SWP
CSB-EP466477SWP
CSB-BP466477SWP
CSB-MP466477SWP
CSB-EP466477SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Probable rRNA maturation factor YbeY (ybeY) CSB-YP466478SWP
CSB-EP466478SWP
CSB-BP466478SWP
CSB-MP466478SWP
CSB-EP466478SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum D-cysteine desulfhydrase (dcyD) CSB-YP466479SWP
CSB-EP466479SWP
CSB-BP466479SWP
CSB-MP466479SWP
CSB-EP466479SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum tRNA (mo5U34)-methyltransferase CSB-YP466480SWP
CSB-EP466480SWP
CSB-BP466480SWP
CSB-MP466480SWP
CSB-EP466480SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein yebF (yebF) CSB-YP466481SWP
CSB-EP466481SWP
CSB-BP466481SWP
CSB-MP466481SWP
CSB-EP466481SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Chromosome partition protein mukF (mukF) CSB-YP466482SWP
CSB-EP466482SWP
CSB-BP466482SWP
CSB-MP466482SWP
CSB-EP466482SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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