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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Macaca mulatta (Rhesus macaque) C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844MOW1
CSB-EP004844MOW1
CSB-BP004844MOW1
CSB-MP004844MOW1
CSB-EP004844MOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Coatomer subunit zeta-1 (Copz1) CSB-YP005797MO
CSB-EP005797MO
CSB-BP005797MO
CSB-MP005797MO
CSB-EP005797MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Small ubiquitin-related modifier 2 (SUMO2) CSB-YP022949BO
CSB-EP022949BO
CSB-BP022949BO
CSB-MP022949BO
CSB-EP022949BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ubiquitin-fold modifier 1 (UFM1) CSB-YP025560HU
CSB-EP025560HU
CSB-BP025560HU
CSB-MP025560HU
CSB-EP025560HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human AP-1 complex subunit sigma-1A (AP1S1) CSB-YP001866HU
CSB-EP001866HU
CSB-BP001866HU
CSB-MP001866HU
CSB-EP001866HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 14-3-3 protein gamma (YWHAG) CSB-YP026288HU
CSB-EP026288HU
CSB-BP026288HU
CSB-MP026288HU
CSB-EP026288HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mitochondrial import inner membrane translocase subunit Tim10 (TIMM10) CSB-YP023548HU
CSB-EP023548HU
CSB-BP023548HU
CSB-MP023548HU
CSB-EP023548HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Mitochondrial import inner membrane translocase subunit Tim13 (Timm13), partial CSB-YP023549RA
CSB-EP023549RA
CSB-BP023549RA
CSB-MP023549RA
CSB-EP023549RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse 40S ribosomal protein S7 (Rps7) CSB-YP020476MO
CSB-EP020476MO
CSB-BP020476MO
CSB-MP020476MO
CSB-EP020476MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) CSB-YP018503HU
CSB-EP018503HU
CSB-BP018503HU
CSB-MP018503HU
CSB-EP018503HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit (Ppp1cb) CSB-YP018504MO
CSB-EP018504MO
CSB-BP018504MO
CSB-MP018504MO
CSB-EP018504MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calmodulin-1 (Calm1) CSB-YP004445RA
CSB-BP004445RA
CSB-MP004445RA
CSB-EP004445RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Neuronal calcium sensor 1 (NCS1) CSB-YP008983HU
CSB-BP008983HU
CSB-MP008983HU
CSB-EP008983HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 26S protease regulatory subunit 4 (Psmc1) CSB-YP018888RA
CSB-EP018888RA
CSB-BP018888RA
CSB-MP018888RA
CSB-EP018888RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig 26S protease regulatory subunit 8 (PSMC5) CSB-YP018895PI
CSB-EP018895PI
CSB-BP018895PI
CSB-MP018895PI
CSB-EP018895PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Calmodulin (Calm1) CSB-YP004445MO
CSB-EP004445MO
CSB-BP004445MO
CSB-MP004445MO
CSB-EP004445MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 40S ribosomal protein S8 (Rps8) CSB-YP020478RA
CSB-EP020478RA
CSB-BP020478RA
CSB-MP020478RA
CSB-EP020478RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 40S ribosomal protein S15a (Rps15a) CSB-YP020373RA
CSB-EP020373RA
CSB-BP020373RA
CSB-MP020373RA
CSB-EP020373RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ubiquitin-conjugating enzyme E2 G1 (Ube2g1) CSB-YP025453RA
CSB-EP025453RA
CSB-BP025453RA
CSB-MP025453RA
CSB-EP025453RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse 14-3-3 protein epsilon (Ywhae) CSB-YP026287MO
CSB-EP026287MO
CSB-BP026287MO
CSB-MP026287MO
CSB-EP026287MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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