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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Human Cytochrome b-c1 complex subunit 6, mitochondrial (UQCRH) CSB-YP025671HU
CSB-EP025671HU
CSB-BP025671HU
CSB-MP025671HU
CSB-EP025671HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform (Phkg1) CSB-YP017927MO
CSB-EP017927MO
CSB-BP017927MO
CSB-MP017927MO
CSB-EP017927MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Laminin subunit beta-1 (LAMB1), partial CSB-YP012730HU
CSB-BP012730HU
CSB-MP012730HU
CSB-EP012730HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tyrosine-protein kinase Lyn (LYN) CSB-YP013259HU
CSB-BP013259HU
CSB-MP013259HU
CSB-EP013259HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tropomyosin beta chain (TPM2) CSB-YP024105HU
CSB-EP024105HU
CSB-BP024105HU
CSB-MP024105HU
CSB-EP024105HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 (Pfkfb1) CSB-YP017817RA
CSB-EP017817RA
CSB-BP017817RA
CSB-MP017817RA
CSB-EP017817RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protamine-2 (Prm2) CSB-YP018729MO
CSB-EP018729MO
CSB-BP018729MO
CSB-MP018729MO
CSB-EP018729MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Inhibin alpha chain (INHA) CSB-YP011718BO
CSB-BP011718BO
CSB-MP011718BO
CSB-EP011718BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken 5-aminolevulinate synthase, nonspecific, mitochondrial (ALAS1), partial CSB-YP001559CH
CSB-EP001559CH
CSB-BP001559CH
CSB-MP001559CH
CSB-EP001559CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ribonuclease pancreatic (RNASE1) CSB-YP019789HU
CSB-EP019789HU
CSB-BP019789HU
CSB-MP019789HU
CSB-EP019789HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Acrosin (ACR) CSB-YP001183PI
CSB-EP001183PI
CSB-BP001183PI
CSB-MP001183PI
CSB-EP001183PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Glutathione S-transferase Mu 2 (Gstm2) CSB-YP009981RA
CSB-EP009981RA
CSB-BP009981RA
CSB-MP009981RA
CSB-EP009981RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Gap junction beta-1 protein (Gjb1), partial CSB-YP009451RA1
CSB-EP009451RA1
CSB-BP009451RA1
CSB-MP009451RA1
CSB-EP009451RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger protein 2 (Zfp2) CSB-YP026444MO
CSB-EP026444MO
CSB-BP026444MO
CSB-MP026444MO
CSB-EP026444MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transcription factor Sp1 (SP1) CSB-YP022438HU
CSB-EP022438HU
CSB-BP022438HU
CSB-MP022438HU
CSB-EP022438HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Neprilysin (MME), partial CSB-YP014653RB1
CSB-EP014653RB1
CSB-BP014653RB1
CSB-MP014653RB1
CSB-EP014653RB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Troponin I, cardiac muscle (TNNI3) CSB-YP024013BO
CSB-EP024013BO
CSB-BP024013BO
CSB-MP024013BO
CSB-EP024013BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Glucose-6-phosphate isomerase (GPI), partial CSB-YP009717PI
CSB-EP009717PI
CSB-BP009717PI
CSB-MP009717PI
CSB-EP009717PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Lactotransferrin (Ltf) CSB-YP013229MO
CSB-EP013229MO
CSB-BP013229MO
CSB-MP013229MO
CSB-EP013229MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Clathrin light chain B (Cltb) CSB-YP005592RA
CSB-EP005592RA
CSB-BP005592RA
CSB-MP005592RA
CSB-EP005592RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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