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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prosthecochloris aestuarii Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP465603EYX
CSB-EP465603EYX
CSB-BP465603EYX
CSB-MP465603EYX
CSB-EP465603EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP465604EYX
CSB-EP465604EYX
CSB-BP465604EYX
CSB-MP465604EYX
CSB-EP465604EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Transcription elongation factor GreA (greA) CSB-YP465605EYX
CSB-EP465605EYX
CSB-BP465605EYX
CSB-MP465605EYX
CSB-EP465605EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP465606EYX
CSB-EP465606EYX
CSB-BP465606EYX
CSB-MP465606EYX
CSB-EP465606EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Aspartate carbamoyltransferase (pyrB) CSB-YP465607EYX
CSB-EP465607EYX
CSB-BP465607EYX
CSB-MP465607EYX
CSB-EP465607EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L19 (rplS) CSB-YP465608ETB
CSB-EP465608ETB
CSB-BP465608ETB
CSB-MP465608ETB
CSB-EP465608ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP465609ETB
CSB-EP465609ETB
CSB-BP465609ETB
CSB-MP465609ETB
CSB-EP465609ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Threonine--tRNA ligase (thrS), partial CSB-YP465610ETB
CSB-EP465610ETB
CSB-BP465610ETB
CSB-MP465610ETB
CSB-EP465610ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Transcription elongation factor GreA (greA) CSB-YP465611ETB
CSB-EP465611ETB
CSB-BP465611ETB
CSB-MP465611ETB
CSB-EP465611ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 30S ribosomal protein S3 (rpsC) CSB-YP465612ETB
CSB-EP465612ETB
CSB-BP465612ETB
CSB-MP465612ETB
CSB-EP465612ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L36 (rpmJ) CSB-YP465613ETB
CSB-EP465613ETB
CSB-BP465613ETB
CSB-MP465613ETB
CSB-EP465613ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Nitrogenase iron protein (nifH) CSB-YP465614ETB
CSB-EP465614ETB
CSB-BP465614ETB
CSB-MP465614ETB
CSB-EP465614ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme ATP phosphoribosyltransferase (hisG) CSB-YP465615ETB
CSB-EP465615ETB
CSB-BP465615ETB
CSB-MP465615ETB
CSB-EP465615ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Protease HtpX homolog (htpX), partial CSB-YP465616ETB1
CSB-EP465616ETB1
CSB-BP465616ETB1
CSB-MP465616ETB1
CSB-EP465616ETB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Cytidylate kinase (cmk) CSB-YP465617ETB
CSB-EP465617ETB
CSB-BP465617ETB
CSB-MP465617ETB
CSB-EP465617ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Shikimate dehydrogenase (aroE) CSB-YP465618ETB
CSB-EP465618ETB
CSB-BP465618ETB
CSB-MP465618ETB
CSB-EP465618ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Dihydroxy-acid dehydratase (ilvD), partial CSB-YP465619ETB
CSB-EP465619ETB
CSB-BP465619ETB
CSB-MP465619ETB
CSB-EP465619ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP465620ETB
CSB-EP465620ETB
CSB-BP465620ETB
CSB-MP465620ETB
CSB-EP465620ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Anthranilate phosphoribosyltransferase (trpD) CSB-YP465621ETB
CSB-EP465621ETB
CSB-BP465621ETB
CSB-MP465621ETB
CSB-EP465621ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme NH (3)-dependent NAD (+) synthetase CSB-YP465622ETB
CSB-EP465622ETB
CSB-BP465622ETB
CSB-MP465622ETB
CSB-EP465622ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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